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《Oceanologia Et Limnologia Sinica》 1995-01
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MEASURING METHODS AND VARIATIONS OF SOME HAEMOLYMPH FACTORS IN PEN AEUS CHINENSIS AFTER THEIR ORAL INGESTION OF IMMUNO DRUGS

Wang Lei; Li Guangyou; Mao Yuanxing(Institute of Oceanology, Academia Sinica, Qingdao 266071)  
From April to September, 1991, the single radial immunodiffusion (SRID) method was applied to determine the haemolymph factors in wild parent prawnsand cultured ones, including deseased ones, the phenoloxidase (PO) activity wasmeasured and its features studied after immuno drugs were orally ingested by theprawns. The results are given below.1. IgM-like substances existed in the haemolymph (which was most probablythe primitive form of IgM in mammals); PO existed in haemocytes in the form ofproenzyme and also exhibited activity in the normal serum of the prawns.2. After three kinds of natural drugs (extracted from land and marine plants)were taken orally by the cultured prawns, the content of haemolymph factors increased and PO activity decreased. The immune system of P. chincnsis could apparentlybe activated by two of those oral immuno drugs.3. SRID determination of IgM-like substances and measurement of PO activitymay be used as references to compare the immuno function of prawns.
【Fund】: 中国科学院“八五”重大应用研究项目!A08920813
【CateGory Index】: S945
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