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《Oceanologia Et Limnologia Sinica》 1995-06
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Li Guangyou; Wangqing(Institute of Oceanology, Academia Sinica, Qingdao 266071)  
This paper reports studies on the morphology, submicroscopic structure and thequantitive method of testing phagocytic activity of P. chinensis collected in Qingdaocoast from March 1991 to April 1992.Results showed that P. chinensis has three distinct populations of circulatinghaemocytes: hyaline cells, semigranu1ar cells, and granular cells; the hyaline cells areround without cytoplasmic granules inside; the semigranulsr cells and the granularcel1s are ovold or ellipsoid and contain cytoplasmic granules; the semigranular cellsplay critical roles in recognizing foreigs objects and have plenty of mitochondria,which is perhaps related to their sensitivity to foreign particles and easy degranula-tion. Golgi apparatus probably associated with the forming of granules can be seenin the granular cells.Gimesa stain results showed that the above three kinds of cells were purplishred, and the nuclei and granules were much deeper, purplish blue, and their beingsimilar to human granular cells. That indicates they have similar functions in hostdefence reactions.The Luminol-enhanced-chemiluminescen (CL) method widely applied in stu-dying phagocytosis kinetics wasused to test phagocytic activity of haemocytes quan-titively. The result of CL response, counts per minute (cpm), can be read from aBeckman scintillation counter. The cpm of the haemocytes mixed with lipopolysac-charide (LPS) and Vibrio suspension were higher than the cpm of haemocyteswith only Vibrio suspension and the cpm of the conttol. It is suggested that LPScan stimulate and enhance the phagocytosis bf blood cells in P. chinensis. Zymosaninduced wear phagocytosis. This method can give quantitve criterion to monitor theimmune function of prawns and will have many applications in aquaculture.
【Fund】: 中国科学院“八五”重大项目!A0892081312
【CateGory Index】: Q952
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