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STUDY ON THE CHANGES OF SOME FACTORS IN HAEMOLYMPH OF INDUCED HALIOTIS DISCUS HANNAI

Ding Xiuyun; Li Guangyou; Zhai Yumei(Department of Bioloyg,Shandong University, Jinan 250100)(Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071)  
Speciments of Haliotis discus Hannai were collected from Rongcheng,Shandong Province in November 1995. The levels of antibacterial, acteriolytic,phenoloxidase and superoxide dismutase (SOD) activities and the blood cell number in the haemolymph of Haliotis discus Hannai were tested at 5, 18, 32, 50h after injection with filtered seawater (conirolled group), with E coli, and with Vibrio sp.The results showed that those infected with E. coli had significant elevations in bacteriolytic activity in the serum at 32h after injection: those infected with Vibrio sp.had the highest level at 5h after injection. Ths proved that the two kinds of bacteria could induee elevations in bpeteriolytic aCtivity in the haemolymph of Haliotis discus Hannai. It is suggested that infeetion with Vibrio sp. and E. coli could induce elevations in antibacterial activity and blood cell number, but that the effeCt of infection with the two kinds of bacteria on phenoloxidase and SOD activities was not obvious. All these proved that proper stimulus can increase the immune protency of H. discus Hannai.
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