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《Oceanologia Et Limnologia Sinica》 1996-06
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THE SPECIFIC EXPRESSION OF ISOZYMES IN DIFFERENT TISSUES AND PRELIMINARY BIOCHEMICAL GENETIC ANALYSIS BASED ON THE ELECTROPHORESIS OF FIVE MARINE FISHES

Wang Keling; You Feng; Xu Cheng; Wu Suqi(Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071)  
Sixty-three fishes belonging to 5 different species (Pagrusomus major, Sparus macrocephalus, Paralichthys olivaceus, Lateolabrax Japonicus, Pseudosciaena polyactis) were collected from the coastal waters of Shandong and Fujian provinces from July to December in 1994. Biochemical samples were extracted by homogenizing from seven tissues (muscle,eye, liver, heart, gill, fin and kidney) of each species separately. Horizontal starch gel and ventical polyacrylamide gel electrophoresis were used in examining 692 samples. Four kinds of buffer systems, TC1, TC2 and EBT for starch gel and TG for polyacrylamide gel electrophoresis, were tested. Altogether 22 isozymes and a prowin were examined through different kinds of hssue or different kinds of buffer systems. only EST, Pm, and SOD were electrophoresed by polyaCrydride ge, the others by hawh gel The results showed the isozymes expedon was twhiy hssue specific. Among the buffer systems tested, TC1, EBT and TG, seemed very useful for the future applicahon due to thdr good resoinhon of isozymes and a protrin, Which eAnbital stable expression and high level of adivity in four kinds of hssue whthe, eye, liver and hcart) during the forphotals (Tab. 1).Furthennre, two buffer systems supporting the stareh ge electrophoresis gave good resolu tion of 12 isozymes: TC1 to LDH, MDH, IDHP and MEP; EBT to ADH, SDH,G6PDH, CAT, GDH, PGM, PGDH, and GPI. Another buffer sydri, TG Was a superior to EST, Pm and SOD for polyacrylamide gel electrophoresis (Tab.2, Tab.3). Finally, based on the zymograms, five isozymes, e.g. LDH, IDHP, ADH, GDH and EST (Plate I), were analynd genhcally on thdr subunit structure, coding gene led and nUmbers as Well as thdr specific expression in there five marine fishes.
【Fund】: 国家科委攀登计划B项目!PDB6-5-2
【CateGory Index】: Q55
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