Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《OCEANOLOGIA ET LIMNOLOGIA SINICA》 1999-02
Add to Favorite Get Latest Update

BIOCHEMICAL GENETIC ANALYSIS OF ISOZYMES ON THE LEFT-EYED FLOUNDER, PARALICHTHYS OLIVACEUS (T.ET S.) IN THE COASTAL WATERS OF SHANDONG

YOU Feng; WANG Ke-ling, XIANG Jian-hai, XU Cheng, WU Su-qi(Institute of Oceanology, ne Chinese Acadeny of Sciences, Qingdao, 266071)  
131 live Ieft-eyed flounders, Paralichlhys olivaceus (T. et S.), were collected from the coastri waters of Qngclao and a fishing farm in Rongcheng City, Shandong Province from May in 1996 to April in 1998. In total, 710 biochemical samples from 6 tissues and organs (eye, muscle, heart, liver, pectoral fin and kidney) were fetched and stored immediately in liquid nitrogen (-196 ℃). The supematant of samples to be used for the experiments were extracted by homogenizing and centrifuging. 15 isozymes (LDH, MDH, MEP, IDHP, G3PDH, AK, CK, ACP, PGM, SDH, ADH, CAT, SOD, GDR GPI) in 3 buffer systems (TC, pH = 6.9; EBT, pH = 8.9; TG pH = 8.3) were tested by horizontal sath gel and vertical POlyacrylthede ge1 electroporesis, and their expression in 6 tissues and organs-tissues specify was also tested. The biochendcal genetics were analyzed in terms of their locus number, structure, alleles, etc., on the basis of which the basic electrophoretic patterns of these l5 isozymes were gained. The electrophoretic patterns of the rest isozymes were not recorded. Altogether 29 gene loci were recorded, among which 9 gene loci including Ldh-A (alleles, 100, 167), Ldh-C (alleles, 98, 100), Idhp-1 (alleles, 78, 100), Acp-1 (alleles, 78, 100), Pgm (alleles, 100, 200), an (alleles, 100, 125), Adh (alleles, 70, 100, 150), Cal (alleles, 93, 100 , 140) and Gdh (alleles, 100, 130) are Polymorph, and the mean proportion of polymorph loci was 31.03%. In the meanhme, the expression of ldh-A and Ldh-B during the biochemical genetic analysis of LDH on Paralichlhys olivaceus (T. et S.), the interrelationships between the activities of isozymes protein and stored condition of samples, and testing of effective genetic markers were also discussed.
【Fund】: 国家攀登计划B资助!PD B-6-5-2
【CateGory Index】: Q178.53
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 MENG Peng1,2,WANG Wei-Ji1,KONG Jie1,LI Xiao-Jing1,2,SHI Jian-Quan31.Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,Shandong,China2.Dalian Fisheries University,Dalian 116023,Liaoning,China3.The Rescues Center of Qinghai Lake-Naked Carp,Xining 810016,Qinghai,China;Isozyme variation of Lake Qinghai naked carp Gymnocypris przewalskii(Kessker)from five rivers[J];Acta Zoologica Sinica;2007-05
2 WANG Dong-qun~1,LI Tai-wu~1,SU Xiu-rong~1,YANG Qin-zhi~2 (1.Agriculture Bureau of Cixi city, Ningbo 315300,China;2.Faculty of life Science and Biotechnology, Ningbo University, Ningbo 315211,China);Factors Affecting Isozymes[J];Fisheries Science;2004-05
3 GAO Yue-mian,LI Guo-xi(School of Life Science and Technology, Dalian Fisheries Univ., Dalian 116023, China);Biochemical genetic analyze of isozymes from Patinopecten yessoensis[J];Journal of Dalian Fisheries University;2003-04
4 XU Cheng,\ WANG Ke Ling, ZHANG Pei Jun (Institute of Oceanology,The Chinese Academy of Sciences,Qingdao,266071);BIOCHEMICAL GENETICS OF LATEOLABRAX JAPONICUS POPULATION Ⅱ.BIOCHEMICAL GENETIC STRUCTURE AND VARIATION[J];Oceanologia Et Limnologia Sinica;2001-03
5 YOU Feng,\ WANG Ke-Ling, XIANG Jian-Hai, XU Cheng (Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071);COMPARATIVE ANALYSIS OF BIOCHEMICAL GENETIC STRUCTURE AND VAR IANCE BETWEEN NATURAL AND CULTURED STOCKS ON THE LEFT-EYED FLOUNDER, PARALICHTHYS OLIVACEUS (T.& S.) OFF SHANDONG COASTAL WATERS[J];Oceanologia Et Limnologia Sinica;2001-05
6 XU Cheng, WANG Ke Ling, XU Yong Li, ZHANG Pei Jun (Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071);RECOMBINATION AND EXPRESSION OF PATERNAL GENE OF ISOZYMES IN GYNOGENETIC OLIVE FLOUNDER PARALICHTHYS OLIVACEUS[J];Oceanologia Et Limnologia Sinica;2002-01
7 WANG Zhi Zheng,(Zhejiang Ocean University,Zhoushan,316004) LI Tai Wu(Life Science and Biotechnology School,Ningbo University,Ningbo,315211) LIU Yan(Life Science School,Liaoning Normal University,Dalian,116029) SUN Xiu Qin(First Institute of Oceano;BIOCHEMICAL GENETIC ANALYSIS OF SIX ISOZYMES ON CHLAMYS FARRERI[J];Oceanologia Et Limnologia Sinica;2002-03
8 MO Zhao Lan, MAO Yun Xiang , CHEN Shi Yong, ZHANG Zhen Dong XU Yong Li, ZHANG Pei Jun (Key Laboratory of Experimental Marine Biology, The Chinese Academy of Sciences; Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071) (Marine Life of Sciences, China Ocean University, Qingdao, 266003);IDENTIFICATION AND PHYLOGENETIC ANALYSIS OF ONE PATHOGENIC BACTERIUM ASSOCIATED WITH SWOLLEN ABDOMEN OF CULTURED FLOUNDER (PARALICHTHYS OLIVACEUS) LARVAE[J];Oceanologia Et Limnologia Sinica;2003-02
9 CHEN Shi-Yong,ZHANG Pei-Jun ,MO Zhao-Lan ,ZOU Yu-Xia ,ZHANG Zhen-Dong ,XU Yong-Li  (Experimental Marine Biology Laboratory,Institute of Oceanology,Chinese Academy of Sciences,Qingdao,266071;Graduate School,Chinese Academy of Sciences,Beijing,100039)  (Experimental Marine Biology Laboratory,Institute of Oceanology,Chinese Academy of Sciences,Qingdao,266071);THE IMPACT OF CULTURE CONDITIONS ON VIBRIO ANGUILLARUM STRAIN M3 GROWTH AND PROTEASE PRODUCTION[J];Oceanologia Et Limnologia Sinica;2004-02
10 WANG Chun Lin, MU Chang Kao , DING Ai Xia , WANG Wu  (Faculty of Life Sciences and Biotechnology, Ningbo University, Ningbo, 315211; Faculty of Life Sciences and Technology, Shanghai Fisheries University, Shanghai, 200090)  (Faculty of Life Sciences and Biotechnology, Ningbo University, Ningbo, 315211)  (Faculty of Life Sciences and Technology, Shanghai Fisheries University, Shanghai, 200090);TISSUE SPECIFICITY AND BIOCHEMICAL GENETIC ANALYSIS OF ISOZYME ON CRUSTACEAN ORATOSQUILLA ORATORIA[J];Oceanologia Et Limnologia Sinica;2004-03
China Proceedings of conference Full-text Database 1 Hits
1 WANG.Ling-Ling~①② SONG Lin-Sheng~① LI Hong-Lei~① XU Wei~① LI Jun-Qiang~① CHANG Ya-Qing~③ (①Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071; ②Graduate School,Chinese Academy of Sciences,Beijing 100039; ③Dalian Fisheries College,Dalian 116023,China);Comparison of Genetic Diversity between Populations of Chlamys farreri Revealed by AFLP and RAPD Analysis[A];CHINESE SOCIETY FOR OCEANOLOGY AND LIMNOLOGY (缩写CSOL)[C];2001
【Citations】
Chinese Journal Full-text Database 3 Hits
1 Wang Keling; You Feng; Xu Cheng; Wu Suqi(Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071);THE SPECIFIC EXPRESSION OF ISOZYMES IN DIFFERENT TISSUES AND PRELIMINARY BIOCHEMICAL GENETIC ANALYSIS BASED ON THE ELECTROPHORESIS OF FIVE MARINE FISHES[J];Oceanologia Et Limnologia Sinica;1996-06
2 Zhu Lanfei(Institute of Hydrobiology, Academia Sinica);COMPARATIVE STUDIES ON LACTATE DEHYDROGENASE ISOZYMES IN SOME CYPRINID FISHES AND THEIR HYBRIDS[J];Acta Hydrobiologica Sinica;1982-04
3 Wu Lizhao Wang Zuxiong(Institute of Hydrobtology,Academia Sinica Wuhan);Study on the Developmental Genetics of Isozymes in Grass Carp (Ctenopharyngodon idellus) Ⅰ. Analysis of Isozymes in Various Tissaes and Organs of Grass Carp[J];Acta Genetica Sinica;1987-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Wu Tingzhang Xu Jinlin Ma Fei Guo Youg (Department of Biology);Comparative Study on LDH and EST Isozymes between Male and Female of Monopterus Albas[J];Journal of Anhui University(Natural Sciences);1992-04
2 Liu Henlie, Zhan guangqin, Wen Hen,(Department of Foundamental Sciences);CHARACTERISTIC ANALYSIS OF ESTERASE ISOZYMES IN GERMINATING RAPE OILSEEDS[J];Journal of Anhui Agricultural University;1990-03
3 Zhang Zhi;Xie Zhongwen Department of Foundamental Sciences;Resource Distribution and Peroxidase Isozyme of Gynostemma in Anhui[J];Journal of Anhui Agricultural University;1993-04
4 Ling Erjun Xu Hourong (Department of Sericultrue,Anhui Agricultural University,Hefei 230036) Yang Sheng quang (Environmental Protection Bureau,Feixi County) Fu Jietao (Agricultural Techonology Centre,Xiaomiao Township);Gel Electrophoresis Studies on Blood Proteins of Bombyx mori with Different Feeding and in Growth Stages[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;1998-01
5 PENG Ren-hai ZhANG Guo-qiang LU Quan-wei (Anyang Institute of Technology, Anyang 455000,China);The Study of Isozymes in Cyprius Carpiovar Singuonesis[J];Journal of Anyang Institute of Technology;2006-02
6 LIU RDSTJN QIN JUNDE (Institute of Zoology, Academia Sinica);A COMPARISON OF THE EGG-WHITE PROTEINS IN THE GENUS ANAS——AN ATTEMPT TO TRACE THE ORIGINATION OF THE PEKING DUCK[J];Acta Zoologica Sinica;1979-03
7 ZHAO ERMI WU GUANFU(Chengdu Institute of Biology, Academia Sinica)WU XIANGFU CHEN YUANCHUNG (Shanghai Institute of Biochemistry, Acaclemia Sinica)JIANG MINGSHI ZHANG JINgKANG HSU KE (Shanghai Institute of Physiology, Academia Sinica);A COMPARISON OF SNAKE VENOM ELETROPHORETOGRAM OF AGKISTRODON SPECIES IN CHINA AND THEIR VALUE IN SNAKE TAXONOMY[J];Acta Zoologica Sinica;1981-03
8 LIU Yong HONG Xijun (Dept. of life science, Southwest Normal University \ \ Chongqing\ 630715);A PRELIMINARY STUDY ON ISOZYME IN SILURUS SOLDATOVI MERIDINALIS AND SME 1 CELL LINE[J];CHINESE JOURNAL OF ZOOLOGY;1997-04
9 Wang Jingxing (Institute of Blood Transfusion,Chinese Academy of Medical Sciences);Pasteurization of Human plasma α_2-Macroglobulin Preparation[J];Chinese Journal of Blood Transfusion;1990-04
10 Wan Shanxia; Qin Ling; Yu Tongchuan(Beijing Agricultural College, Bejing 102206);Effect of Water Stress on POD SOD Activity and iso-POD SOD in Chinese Chestnut Seedlings Leaves[J];JOURNAL OF BEIJING AGRICULTURAL COLLEGE;1997-03
China Proceedings of conference Full-text Database 2 Hits
1 Zhang Jie Chen Liqiao Zhou Zhongliang Tang Sixian;Study on the Developmental Genetics of Isozymes in Chinese Mitten-handed Crab I.Discussion on Isozymes in Various Tissues of Chinese Mitten-handed Crab from Changjiang River[A];[C];1996
2 WANG Gui-ling, XU Hang, CHEN Zhi-guo, SHEN Wei-rong College of Aqua - life Science and Technology, Shanhai Fisheries University, Shanghai, ,200090 School of life science, Ningxia University, Yinchuan 750021;Studies on four isozyme systems in sex reversal process of Monopterus albus[A];[C];2005
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Zhang Xiaofei et al (Institute of Animal Husbandry and Veterinary Medicine, AAAS, Hefei,Anhui 230031);Dynamic Changes of Serum Biochemical Indexes on Experimental Infected Ducklings with Duck Hepatitis Virus[J];Journal of Anhui Agricultural Sciences;2004-04
2 WU Jia xi 1,ZHAO Zheng long 1,XU Xiao feng 2 (1.Department of Sericulture and Silk Textile,Anhui Agricultural University,Hefei 230036; 2.College of Life Sciences,Nanjing Normal University)$$$$;Study on Peroxidase Isoenzyme Bands and Activity of Some Mulberry Varieties[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;2001-03
3 WAN Quan 1,LIU En sheng 1,SHENG De ling 2,TANG Hui qing 1,XUE Ming 1,LI Yan he 1 (1 Department of Fishery,Anhui Agricultural University,Hefei 230036; 2 Fisheries Station of Anhui Province);Analysis on Karyotype of Leiocassis longirostris Günther[J];Journal of Anhui Agricultural University;2002-02
4 MIAO Jin lai 1, 2 , SHI Hong qi 1, 2 , LI Guang you 2, WANG Bo 2 & HAN Li 2 (1 Marine Life College, Ocean University of Qingdao, Qingdao 266003, China) (2 Key Laboratory of Marine Bio active Substances, State Ocean Admission, Qingdao 26;SOME RESEARCH ON THE WAYS OF RED TIDE PREDICTION AND PREVENTION[J];Journal of Safety and Environment;2002-03
5 TCHANG-SI(Institut d'oceanologie, Academia Sinica);SURLAPRESENCE DU GENRE ASYMMETRON DANS LA MER DE CHINE ET LA DISTRIBUTION GEOGRAPHIQUE DE BRANCH10STOMA BELCHERI (GRAY)[J];Acta Zoologica Sinica;1962-04
6 CHU SINLUO CHEN YINBUI (Kunming Institute of Zoology, Aeademia Sinica);A NEW BLIND COBITID FISH (PISCES, CYPRINIFORMES) FROM SUBTERRANEAN WATERS IN YUNNAN, CHINA[J];Acta Zoologica Sinica;1979-03
7 ZHANG XIAOWEI, He Guifen and Sha Xueshen (Institute of Oceanology, Academia Sinica);MORPHOLOGICAL STUDIES OF THE EGGS,LARVAE AND YOUNG FISH OF THE BLACK PORGY,SPARUS MACROCE-PHALUS (BAS1LEWSKY)[J];Acta Zoologica Sinica;1980-04
8 ZHANG XIAOWEI , CHEN ZHENRAN ,BUAN HONGCHAO, HE GUIFEN AND SHA XUESHEN(Institute of Oceanology, Academia Sinica);ON THE DEVELOPMENT OF THE EGGS AND LARVAE OF THRISSA KAMMALENSIS AND THRISSA MYSTAX[J];Acta Zoologica Sinica;1982-02
9 XIONG QUANWEI XIA SHENGLIN (Department of Biology, Wuhan University);A STUDY OF ISOZYMES IN MYXOCYPRINUS ASIATICUS (BLEEKER)[J];Acta Zoologica Sinica;1985-01
10 ZHANG CHUNGUANG (Institute of Zoology, Acadetnia Sinica);ON THE ECOLOGICAL ADAPTATION AND GEOGRAPHICAL DISTRIBUTION OF THE BARBINE FISH VARICORHINUS (SCAPHESTHES) MACROLEPIS (BLEEKER)[J];Acta Zoologica Sinica;1986-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 LIU Li-hui et al(Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou,Guangdong 510380);Isolation and Classification of Pathogenic Bacterium Caused by Gill-rote Disease in Channel Catfish(Ictalunes punctatus)[J];Journal of Anhui Agricultural Sciences;2008-17
2 SUN Xiu-Jun et al(Shanghai Fisheries University,College of Life Science and Technology,Shanghai 2000902);Comparative Analysis of Morphological Indices of Papanese Scallops with 2 Shell Colors[J];Journal of Anhui Agricultural Sciences;2008-23
3 YIN Qian-qian1,2,LI Da-yu1,2,WANG Hong-zhe1,2,CAO Ding-chen1,LU Cui-yun1,SUN Xiao-wen1,LIANG Li-qun1 (1.Heilongjiang Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070;2.College of Aqua-life Science and Technology,Dalian Fisheries University,Dalian 116023);Microsatellite marker analysis on genetic diversity in two German mirror carp(Cyprinus carpio L.) families[J];Journal of Anhui Agricultural University;2008-02
4 MENG Peng1,2,WANG Wei-Ji1,KONG Jie1,LI Xiao-Jing1,2,SHI Jian-Quan31.Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,Shandong,China2.Dalian Fisheries University,Dalian 116023,Liaoning,China3.The Rescues Center of Qinghai Lake-Naked Carp,Xining 810016,Qinghai,China;Isozyme variation of Lake Qinghai naked carp Gymnocypris przewalskii(Kessker)from five rivers[J];Acta Zoologica Sinica;2007-05
5 WANG Xin-An1,MA Ai-Jun 1,XU Ke 1,2,LEI Ji-Lin1,YANG Zhi3,QU Jiang-Bo31.Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Science,Key Laboratory for Sustainable Utilization of Marine Fisheries Resource,Ministry of Agriculture,Qingdao 266071,Shandong,China2.College of Aqua-life Science and Technology,Shanghai Fisheries University,Shanghai 200090,China3.Yantai Tianyuan Aquatic limited corporation,Yantai 264003,Shandong,China;Relationship between morphometric attributes and body weight of juvenile turbots Scophthalmus maximus[J];Acta Zoologica Sinica;2008-03
6 WANG Ling Ling ①② SONG Lin Sheng ① LI Hong Lei ① XU Wei\+① LI Jun Qiang ① CHANG Ya Qing ③ (①Institute of Oceanology, Chinese Academy of Sciences, Qingdao\ 266071; ②Graduate School, Chinese Academy of Sciences, Beijing\ 100039; ③Dalian Fisheries College, Dalian\ 116023, China);Comparison of Genetic Diversity between Populations of Chlamys farreri Revealed by AFLP and RAPD Analysis[J];Chinese Journal of Zoology;2003-04
7 Lü Yao-ping1,2,HU Ze-hui3,4,YE Li-ping1(1.College of Chemistry and Life Sciences,Lishui University,Lishui Zhejiang 323000,China;2.College of Animal Science,Zhejiang University,Hangzhou 310029,China;3.Marine Fisheries Research Institute of Zhejiang Province,Zhoushan Zhejiang 316100;4.China Shanghai Fisheries University,Shanghai 200090,China);The Study on Extraction Methods of Total DNA from Hemibarbu Labeo and Optimization of ISSR-PCR[J];Journal of Jiangxi Normal University(Natural Sciences Edition);2009-02
8 MU Chang kao,WANG Chun lin(Faculty of life science and biotechnology,Ningbo University,Zhejiang 315211);The Research Review of the Isozyme in Crustacea[J];Fisheries Science;2003-04
9 WANG Dong-qun,LI Tai-wu,SU Xiu-rong (Life Science and Biotechnology School, Ningbo University, Ningbo 315211,China);Study on Biochemical Genetic Marker of six Populations of Sinonovacula constricta Lamarck[J];Fisheries Science;2004-04
10 DU Tao~1,LUO Jie~1,LIN Xiang-yang~2 (1.Zhanjiang Ocean University, Zhanjiang 524025,China;2.Fujian Fisheries Research Institute, Xiamen 361012,China);Comparison of Artificial Breeding of Marine Fish between Indoor Tanks and Outdoor Ponds[J];Fisheries Science;2004-07
China Proceedings of conference Full-text Database 3 Hits
1 HE Pei-qing,LIU Chen-lin,HU Xiao-ying,CHEN Kao-shan (First Institute of Oceanography, SOA, Qingdao 266061, China; Key Lab of Marine Bioactive Substances, SOA, Qingdao 266061, China; College of Life Sciences, Shandong University, Jinan 250100, China);Effect of Introduced Marine Economic Species on Genetic Diversity of Native Resources in China[A];[C];2005
2 YAO Zong-li, ZHOU Kai, LAI Qi-fang, WANG Hui, XIA Lian-jun (East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences。Key and Open Laboratory of Marine and Estuarine Fisheries, Ministry of Agriculture, Shanghai 200090, China);Analysis of genetic variations of five wild populations in Cyclina sinensis ( Gmelin) of China[A];[C];2005
3 ZENG Shou-ying,YANG Ning-sheng,OUYANG Hai-ying (Research Center of Information and Economy,Chinese Academy of Fishery Sciences,Beijing 100141,China);Advancements in germ plasm of naked carps in Qinghai-lake[A];[C];2008
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 Yangtze River Fisheries Eesearch Institute;AN EXPERIMENTAL REPORT ON BACKCROSS BREEDING OF BIG HEAD (ARISTICHTHYS NOBILIS)♀×[BIGHEAD(A NOBILIS)♀× SILVER CARP (HYPOPHTHALMICHTHYS MOLITRIX♂)]♂[J];Acta Genetica Sinica;1975-02
2 Yang Xingqi, Deng Chuxia, Chen Hongxi (Institute of Hydrobiology, Academia Sinica, Wuhan);Electrophoretic Studies on the Lactate Dehydrogenase and Malate Dehydrogenase Isozymes in Tissues of Three Species in Genus Tilapia[J];Acta Genetica Sinica;1984-02
3 Wu Lizhao Wang Zuxiong(Institute of Hydrobiology, Academia Sinico, Wuhan 430072);Biochemical Genetic Structure and Variation in A Natural Population of Grass Carp from the Middle Reaches of the Yangtze River[J];Acta Genetica Sinica;1992-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved