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《OCEANOLOGIA ET LIMNOLOGIA SINICA》 1999-02
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SENSITIVITY OF SPOROPHYTES OF PARTHENOGENETIC LAMINARIA JAPONICA TO CHLORAMPHENICOL AND HYGROMYCIN

LI Xin-ping; QIN Song; ZENG Cheng-Kui (C.K. Tseng)(Institute of Oceanology, ne Chinese Academy of Sciences, Qingdao, 266071)  
In order to optimize the selection condition for bosgenic Laminaria japonica, the sensitivity of sporophytes of parthenogenehc L japonica to chloramphenicol and hygromycin was tested from Jan. to Apr., 1997. Young sporophytes of Parthenogenetic L japonica were regenerated from Parthenogenetic gamtophytes and cultured in laboratory. The total lengths of young sporophytes were from 0.5 to 1.5cm. In the test for chiormphenicol, the young sporophytes were divided into three groups according to their total lengths: a) (4.9±0.6)mm, b) (6.5±0.5)mm, and c) (8.4±0.9 ) mm. Chloramphenicol concentration in each group was 0.50.0, 65.0, 84.5, 110.0, 143.0, 185.5, and 241.2μg / ml, respechvely. In the test for hygromycin, the young sporophytes were also divided into fore groups i. e. a) (6.3±1.0)mm, b) (8.3±1.0)mm, and c) (12.3±2.3)mm. Hygromycin concentration in each group were 0, 50.0, 65.0, 84.5, 110.0, and 143.0μg / ml, respechvely. Steril polystyrene dishes (diameter 3.0cm) were used, which contalns 51ml enriched seawater added with necessary anhbihcs stOCk. Every ten young sporophytes were Put into a Plate, cultUred at (10.0±0.5) t in about 50μE / (m2 s), 10h light/ 14h darknss, and their death were observed everyday. All media were renewed every week. LD50 of Chloramphehicol at 15, 16, 17, 18, 19 and 20d and LD50 of hygromycin at 36, 48, 72 and 96h were calculated. The results show tha the LD50 of L. japonica to chloramphenicol is correlated with toed length (0.5-1.5cm), but the LD50 of hygromycin is not The Parthnogenetic sporophytes are more sensihve to hygromycin than to chloramphenicol. For exaxnple, LD50 of chlor-amphenicol in the tae groups at 15d are 158.9, 110.6 and 89.0μg /ml, respechvely, but LD50 of hygromycin in the three grouPs at 96h (4d) are only 47.4, 50.7 and 69.4μg/ml, respechvely. This observation snggests that HPH (hygromycin phosphobesferase) gene is a more efficient selectable marker than CAT (chlormphenicol acetyltransferase) gene.
【Fund】: 国家攀登计划B资助!PD B-6-4-1;;国家自然科学基金!39400076
【CateGory Index】: Q789
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