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《OCEANOLOGIA ET LIMNOLOGIA SINICA》 1999-03
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EFFECTS OF ENVIRONMENTAL FACTORS ON SPERM MOTILITY IN FLOUNDER, PARALICHTHYS OLIVACEUS (T. ET S.)

WANG Hong-tian; ZHANG Pei-jun(Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071)  
Between May and June in 1997, experiments were conducted to determine the effects of external factors on the sperm motility in flounder, Paralichthys olivaceus (T. et S.). The different solutes, which included sugar, NaCl, KCl, CaCl2, MgCl2, EDTANa2, were dissolved in double distilled water to produce solutions with different concentrations. The sperm could be initiated by increasing the external osmolity. With different solutes, the osmolity levels that could initiate or terminate the sperm motility were different. EDTANa2 had no induction effect. When EUTANa2was added to the artificial seawater, the sperm could not be initiated and the original active sperm was inhibited. When Ca2+ was dissolved in such solution, the sperms tha were inhibited could not motile again. When MgSO4 was dissolved in the solution to replace CaCl2, the sperm could be initiated. But when CaCl2 was replaced by MgCl2, or MgSO4 was replaced by CaCl2 or NaCl, the sperm kept inactive. The sperm could be induced by the seawater with different pH-value ranging between 4.0 and 9.5.
【Fund】: 国家攀登计划B资助!PDB-6-1-2
【CateGory Index】: X503.2
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