Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《OCEANOLOGIA ET LIMNOLOGIA SINICA》 1999-03
Add to Favorite Get Latest Update

STUDY OF POPULATION GENETIC STRUCTURE IN PENAEUS JAPONICUS WITH RAPD MARKERS

SONG Lin-sheng; XIANG Jian-hai; LI Chen-xi;; ZHOU Ling-hua;LIU Bao-zhong; LIU Rui-yu(Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071)  
In April 1997, samples of natural population and hatchery stock of Penaeus japonicus were collected from Fuian Province and Qingdao, Shandong Province respectively. The RAPD (random amplified polymorphic DNA) technique was applied to the examination of the genetic structure of the natural population and hatchery stock of Penaeus japonicus. Genomic DNA was extracted from 20 individuals for natural population and hatchery stock. Amplification with 20 random primers under predetermined optimal reaction conditions (samples were first heated at 94℃ for 5min, and followed by 45 cycles of 1min. at 94℃, 1min. at 36℃, and 2min. at 72℃, then held at 72℃ for 10min. after 45 cycles) generated 157 and 153 fragments in natural population and hatchery stock, respectively. The polymorphic fragments were 85 and 58, and the mean proportions of polymorphic amplified fragments of natural population and hatchery stock were 54.14% and 37.91%, respectively. The value of average heterozygosity for natural population and hatchery stock were 0.251 7 and 0.134 3, respectively. The higher value of the average heterozygosity of natural population indicates that the natural resomee of Penaeus japonicus was in good condition with higher genetic variability, and effective measare should be carried out to mainnin the good status.The lower heterozygosity in the hatchery stock suggests that the approaches such as marker assisted selection should be induced into the shrimp breeding programs.
【Fund】: 国家自然科学基金!39470141;;国家攀登计划B资助!PDB-6-5-3;;中国科学院院长基金!963054
【CateGory Index】: S968.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 LIU Chen-ying JIANG Hui ( College of life science, Wuhan University, Wuhan 430072);Demonstrating Poorness of Genetic Diversity of Dugesia japonica in Luojia Mountain by RAPD Analysis[J];Amino Acids & Biotic Resources;2005-03
2 WANG Ling Ling ①② SONG Lin Sheng ① LI Hong Lei ① XU Wei\+① LI Jun Qiang ① CHANG Ya Qing ③ (①Institute of Oceanology, Chinese Academy of Sciences, Qingdao\ 266071; ②Graduate School, Chinese Academy of Sciences, Beijing\ 100039; ③Dalian Fisheries College, Dalian\ 116023, China);Comparison of Genetic Diversity between Populations of Chlamys farreri Revealed by AFLP and RAPD Analysis[J];Chinese Journal of Zoology;2003-04
3 SONG Wei-wei~1,WANG Chun-lin~1,MU Chang-kao~2(1.Faculty of Life Science and Biotechnology,Ningbo University,Ningbo 315211, China;2.College of Life Science and Technology of Xiaogan University,Xiaogan 432000, China);Genetic Diversity in Mantis Shrimp Oratosquilla kempi by Isozyme and RAPD Analysis[J];Fisheries Science;2006-04
4 JIANG Ye-qin (School of Life Sciences,Huzhou Teachers College,Huzhou 313000,China);Status quo of the study on the genetic diversity of shrimps[J];Journal of Marine Sciences;2009-01
5 CHANG Lin-rui1,SUN Zhen-xing1,LI Li2,LI Yun-ling1(1.College of Life Science,Ludong University,Yantai 264025,Shangdong,China; 2.College of Life Science,Ocean University of China,Qingdao 266003,Shangdong,China);DNA Extraction and Optimization of RAPD Reaction System for Apostichopus japonicus[J];Hubei Agricultural Sciences;2007-01
6 SU Ying~1,LIU Chu-wu~1,YE Chang-hui~1,LIU Li~1,ZHANG Jin~2,CAI Di-yang~2 (1.Guangdong Ocean University,Zhanjiang 524088,China;2.Zhanjiang Potou Bureau of Animal Husbandry,Zhanjiang 524045,China);RAPD Analysis of the Genomic of Parent Ducks and Their Hybrids[J];Journal of Henan Agricultural Sciences;2006-01
7 ZHANG Junbin1,2, HUANG Hui2, CAI Zeping2, LIU Sheng2, XU Zhanzhou2 ( 1. Institute of Oceanography, Chinese Academy of Sciences, Qingdao 266071, Shandong, China ;2. South China Sea Institute of Oceanography, Chinese Academy of Sciences, Guangzhou 510301, Guandong, China);Research Advances in the Population Genetics of Marine Animals[J];Marine Science Bulletin;2005-06
8 LI Li1, SUN Zhenxing2, CHANG Linrui2, LI Yunling2, ZHANG Mingqing3 ( 1. College of Life Science and Technology, Shanghai Fisheries University, Shanghai 200090, China; 2. College of Life Science, Yantai Normal University, Yantai 264025, Shandong, China; 3. Gaolilai Foodstuff Limited Corporation, Qingdao 266555 ;S handong, China);Genetic Diversity in the Wild and Cultivated Populations of Haliotis discus hannai[J];Marine Science Bulletin;2005-06
9 LI Hong-Lei, SONG Lin-Sheng,LIU Bao-Zhong, CUI Zhao-Xia, XIANG Jian -Hai (Institute of Oceanology,The Chinese Academy of Sciences,Qingdao,266071) LIU Sheng-Ping, QU Shan-Cun, JIANG Guang-Liang (Aquiculture Technique Exploitation Center of Yantai,Yantai;STUDIES ON THE GENETIC STRUCTURE OF DIFFERENT POPULATION OF CHLAMYS FARRERI AND THEIR HYBRIDS′ HETEROSIS[J];Oceanologia Et Limnologia Sinica;2002-02
10 ZHANG Guo Fan, WANG Ji Hong , ZHAO Hong En  , QUE Hua Yong, LIU Xiao (Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071)  (Dalian Fisheries University, Dalian, 116023)  (Dalian In;THE RAPD MARKER OF SELF-BRED AND HYBRID PROGENY BETWEEN CHINESE AND JAPANESE POPULATIONS OF HALIOTIS DISCUS HANNAI INO[J];Oceanologia Et Limnologia Sinica;2002-05
China Proceedings of conference Full-text Database 1 Hits
1 WANG.Ling-Ling~①② SONG Lin-Sheng~① LI Hong-Lei~① XU Wei~① LI Jun-Qiang~① CHANG Ya-Qing~③ (①Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071; ②Graduate School,Chinese Academy of Sciences,Beijing 100039; ③Dalian Fisheries College,Dalian 116023,China);Comparison of Genetic Diversity between Populations of Chlamys farreri Revealed by AFLP and RAPD Analysis[A];CHINESE SOCIETY FOR OCEANOLOGY AND LIMNOLOGY (缩写CSOL)[C];2001
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 XU Jian-peng et al(College of Life Science,Ludong University,Yantai,Shandong 264025);DNA Extraction from Kareius bicoloratus Genome and Its Optimization of RAPD Reaction System[J];Journal of Anhui Agricultural Sciences;2008-35
2 RAO Long-bing(Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Fuyang,Zhejiang 311400);Discussion on the Key Problems in the Research of Genetic Diversity by Molecular Markers[J];Journal of Anhui Agricultural Sciences;2009-15
3 LIU Chen-ying JIANG Hui ( College of life science, Wuhan University, Wuhan 430072);Demonstrating Poorness of Genetic Diversity of Dugesia japonica in Luojia Mountain by RAPD Analysis[J];Amino Acids & Biotic Resources;2005-03
4 WANG Wei-Ji1,2,ZHANG Tian-Shi2,YANG Cui-Hua1,2,LUAN Sheng2,KONG Jie2,WANG Qing-Yin21.College of Life Science and Technology,Ocean University of China,Qingdao 266003,Shandong,China2.Key Laboratory for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,Shandong,China;QTL associated with WSSV-resistance and growth-related traits in the Chinese shrimp Fenneropenaeus chinensis[J];Acta Zoologica Sinica;2008-06
5 WANG Ling Ling ①② SONG Lin Sheng ① LI Hong Lei ① XU Wei\+① LI Jun Qiang ① CHANG Ya Qing ③ (①Institute of Oceanology, Chinese Academy of Sciences, Qingdao\ 266071; ②Graduate School, Chinese Academy of Sciences, Beijing\ 100039; ③Dalian Fisheries College, Dalian\ 116023, China);Comparison of Genetic Diversity between Populations of Chlamys farreri Revealed by AFLP and RAPD Analysis[J];Chinese Journal of Zoology;2003-04
6 WU Ai-Chun~① ZHANG Yong-Pu~ ②③ ZHOU Hua-Bin~③(① Yongjia College of Zhejiang Radio and TV University, Wenzhou 325100;② School of Life and Environmental Sciences, Wenzhou University, Wenzhou 325027;③ Research Institute of Biological Resource and Biodiversity, Wenzhou University, Wenzhou 325027, China);Analysis and Evaluation of the Nutritive Components in the Edible Part of Estellarca olivacea[J];Chinese Journal of Zoology;2009-01
7 ZHANG Ji-ping~1, HE Shun-lian~1,ZHOU Meng-jiao~2, GUO Jin-biao~1, ZHANG Qi-qiong~1 (1.Department of Animal Science, Foshan Science and Technology College, Foshan 528231,China; 2.College of Animal Science,Hunan Agriculture University, Changsha 410128,China);The Selection of Polymorphic Primers to Three Species of Monopterus by RAPD Analysis[J];Fisheries Science;2004-09
8 LI Cheng-Hua~(1,2,3), LI Tai-Wu~2, SONG Lin-Sheng~1, SU Xiu-Rong~2 (1. Experimental Marine Biology Laboratory, Institute of Oceanology, Chinese Academy of Science, Qingdao 2660711; 2. Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211; 3. Graduate Student Faculty of Chinese Academy of Science, Beijing 100039);Genetic Variations Among Four Populations of Sinonovacula constricta by Using Random Amplified Polymorphic DNA[J];Fisheries Science;2004-12
9 ZHANG Yu-yong, CHANG Ya-qing, SONG Jian ology, Ministry of Agriculture, People's Republic of China, Dalian 116023, China);Application and Recent Research Advancement of Cross-breeding in Marine Shellfish Culture[J];Fisheries Science;2005-04
10 PEI Ying,WANG Xiao-hong,ZHANG Heng-qing(College of Life Science,Liaoning Normal University,Dalian 116029,China);RAPD Analysis for Ruditapes philippinarum Along Dalian Coast of Bohai Sea[J];Fisheries Science;2006-05
China Proceedings of conference Full-text Database 9 Hits
1 LIU Chen-lin, HE Pei-qing, HU Xiao-ying, HUANG Xiao-hang (First Institute of Oceanography, SOA, Qingdao 266061, China; Key Lab of Marine Bioactive Substances, SOA, Qingdao 266061, China);Application of Molecular Genetic Marker Technique in Identifying the Organism Invasions in Coastal Zone[A];[C];2005
2 HAO Lin-hua, SHI Hong-qi, WANG Neng-fei, CHEN Kao-shan (First Institute of Oceanography, SOA, Qingdao 266061, China; Key Lab of Marine Bioactive Substances , SOA, Qingdao 266061, China; College of Life Sciences, Shandong University, Ji'nan 250100, China);Present Status and Ecological Harming of Alien Marine Organism Invasion[A];[C];2005
3 LI Fa-jun~1,FU Hong-Tuo~(1,2),QIAO Hui~1,LI Ming-shuang~1 (1.Wuxi Fishery College,Nanjing Agricultural University,Wuxi 214081,China;2.Freshwater Fishery Research Center,Chinese Academy of Fishery Sciences,Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology,Ministry of Agriculture,Wuxi 214081,China);Analysis of genetic diversity among Macrobrachium nipponensis populations in the Yangtze River using microsatellite markers[A];[C];2008
4 LI Fa-jun~1,FU Hong-tuo~(1,2),QIAO Hui~1,LI Ming-shuang~1 (1.Wuxi Fishery College,Nanjing Agricultural University,Wuxi 214081,China;2.Freshwater Fishery Research Center,Chinese Academy of Fishery Sciences,Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology,Ministry of Agriculture,Wuxi 214081,China);Identification of microsatellite loci by magnetic bead enrichment method in Macrobrachium nipponensis[A];[C];2008
5 YAO Qian~1 YANG Pin~1 CHEN Li-qiao~1 GUO Hui~1 ZHANG Hao~1 YU Na~1 YANG Guo-liang~2 WANG Jun-yi~2 1.School of Life Science,East China Normal University,Shanghai 200062,China; 2.Zhejiang South Tailake Freshwater Fish Breeding Co.,Ltd,Huzhou 313001,China;Interspecific DNA sequence polymorphism in the mitochondrial D-loop gene from three populations of Macrobrachium rosenbergii[A];[C];2006
6 LI Tai-wu JI Yan-bin SU Xiu-rong (Key Laboratory of Applied Marine Biotechnology of Ministry of Education,Ningbo University Ningbo 315211,China);Multivariation analysis of nutritive material of Moerella iridescens along the Zhejiang Coast[A];[C];2006
7 QuYanbo YeHui ShiYaohua WangYan GuZhifeng Wang Aimin Hainan Provincial Key Laboratory for Tropical Hydrobiology & Biotechnology, Ocean College, Hainan University, Haikou, 570228;RAPD Analysis of Genetic Variation among Cross Breeding System of Pearl Oyster[A];[C];2006
8 Ye Hui Qu Yanbo Wang Yan Shi Yaohua Gu Zhifeng Wang Aimin Hainan Provincial Key Laboratory for Tropical Hydrobiology & Biotechnology, Ocean College, Hainan University, Haikou, 570228;RAPD Analysis of Genetic Difference among 8 Family Lines of Pearl Oyster[A];[C];2006
9 LI Zhong-Bao (Fisheries College,Jimei University,Xiamen 361021 China);Comparative study on MtDNA COⅠ and COⅡ gene fragment of Haliotis ovina and H.asinine[A];[C];2006
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved