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《OCEANOLOGIA ET LIMNOLOGIA SINICA》 1999-03
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EFFECTS OF IMMUNOPOIYSACCHARIDE ON LSZ, ALP, ACP AND POD ACTIVITIES OF PENEUSIS SERUM

LIU Shu-qing; JIANG Xiao-lu; MOU Hai-jin; WANG Hui-mi; GUAN Hua-shi(Fishery College, Ocean University of Qingdao, Qingdao, 266003)  
Penaeus chinensis with a body length of 12-14cm were bought from Hongdao prawn culturing farm in Qngdao in September, 1997. The prawns were nursed in the glass tanks in laboratory. Seaweed polysaccharide and Cordyceps militaris polysascharide with various concentration were injected intraperitoneally into the bodies of P. chinensis. Hemolymph was collected by heart puncture from the rear of cephalothorax at 24h, 48h, 72h after the injection. The activities of LSZ,ALP, ACP, POD and the content of total protein in the hemolymph were determined.The results show that after P. chinensis was stimulated by seaweed polysaccharide and Cordyceps militaris polysaccharide, all the activities in the hemolymph increased. Two kinds of immunopolysaccharide could be used as non-special immunopotentiator to enhance the immunological functioning of P. chinensis and its defensive ability to pathogen. This study may represent a new application of immune polysaccharide to the prevention and care of the prawn disease.After the injection of 1.0% seaweed polysaccharide and Cordyceps militaris polysaccharide for 48h, the activities of LSZ increased from 0.25U /ml of the control group to 0.75U/ ml and 0.88U /ml separately, then decreased rapidly at 72h. The activities of ALP enhanced slightly at 24h and 48h,and increased from 1.2U / mg of the control group to 25U / mg and 48U / mg separately at 72h.The activities of ACP increased gradually too, changed from 4.5U/ mg of the control group to 17U / mg and 30U/ mg at 72h. The activities of POD increased to a maximum value at 48h, were 1.96U/ mg and 2.20U / mg from 0.11U / ml of the control group, and dropped a littie at 72h.However, the content of protein in hemolymph was stable. Because of the effects of polysaccharide on the phagocytosis of phagocytes, the activities of LSZ ALP, ACP, POD increased in varying degrees. It shows that these enzyme activities were related closely with the immunity of P. Chinensis and could be used as an index of detecting the prawn's health and immunological competence. As two kinds of immune drugs, seaweed polysaccharide and Cordyceps militaris polysaccharide could be favourable to the activities of LSZ, ALP, ACP in the hemolymph of P. chinensis and could enhance the metabolic capablity. So two kinds of polysaccharide possessed som application value in the prevention and cure of prawn disease.
【Fund】: 国家攀登计划B资助!PDB-6-6-3
【CateGory Index】: S945.19
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【References】
Chinese Journal Full-text Database 10 Hits
1 LIU Zhan-cai et al(Department of Biology,Jiaozuo Teachers College,Jiaozuo,Henan 454001);Effect of Hg~(2+) on Lysozyme Activity in Serum and Kidney of Grass Carp[J];Journal of Anhui Agricultural Sciences;2007-09
2 HE Ying et al (Guangxi Institute of Veterinary,Nanning,Guangxi 530001);Immunoregulation Effect of Sargassum Polysaccharides on White-leg Shrimp[J];Journal of Anhui Agricultural Sciences;2008-31
3 GUAN Jian-yi et al (Department of Life Science,Xinxiang Medical College,Xinxiang,Henan 453003);Effect of Beta-cypermethrin on Lysozyme Activity in Serum of Grass Carp[J];Journal of Anhui Agricultural Sciences;2009-09
4 YAN Jian-guo, RU Shao-guo, WANG Wei(Marine Life College, Ocean University of China, Qingdao 266003, Shandong, China);On effects of monocrotophos on AChE, CaE, ALP and ACP activities of Monopterus albus[J];Journal of Safety and Environment;2006-03
5 SONG Xiu-kai~1,WANG Wei~2,LIU Yun-zhang~2,ZHAO Yuan-yuan~2,RU Shao-guo~2(1 Marine Fisheries Research Institute of Shandong,Yantai 264006,Shandong,China;2 Department of Marine Biology,Ocean University of China,Qingdao 266003,Shandong,China);Effects of physical and chemical factors on the hemolysis of the Anabaena variabilis hemolysin[J];Journal of Safety and Environment;2006-06
6 LI Zhi Hua XIE Song WANG Wei Na WANG Jun Xia (College of Life Sscience,Hebei University,Baoding 071002,China);Toxicity of Nitrite-Na and Sodium Polyphosphate to the Shrimp Macrobrachium nipponense[J];Chinese Journal of Zoology;2004-03
7 ZHOU Xiao qiu 1,KUANG Sheng yao 2,TANG Ling 2 (1 Animal Nutritional Institute of Sichuan Agricultural University,Yaan 625014,China; 2 Sichuan Animal Science Academy,Chengdu 610066,China);Effects of Oligomannose and Fructose Levels on Growth and Transport of Carp[J];Fisheries Science;2002-02
8 LI Guoli1 ,WANG Anli2 ,HU Junrong2,ZHANG Haitao1(1Hebei University, Baoding 071002,China; 2South China Normal University ,Guangzhou 510631,China);The Mechanism and Efficacy of Herbal Medicine on Aquatic Animal Disease Prevention[J];Fisheries Science;2003-04
9 CHEN Yin-er,WANG Guo-liang,JIN Shan,LI Zheng(Faculty of Life Science and Biotechnology,Ningbo University,Ningbo 315211,China);Enzyme Activities in Portunus trituberculatus with Emusification Disease[J];Fisheries Science;2006-09
10 LI Chang-hong,JIN Shan(College of Life Science and Biotechnology,Key Laboratory of Applied Marine Biotechnology,Ministry of Education,Ningbo University,Ningbo 315211,China);Preliminary Study on Immune Function of the Haemolymph in Crab Portunus trituberculatus[J];Fisheries Science;2008-04
China Proceedings of conference Full-text Database 3 Hits
1 HAO Lin - hua, CHEN Kao - shan, QU Jiang - bo, YANG Zhi The 1 st Institute of Oceanography, SOA; Key Lab of Marine Bioactivity Substance of State Oceanic Administration, Qingdao 266061, China; Life Science Institute, Shandong University, Jinan 250100, China; Yantai Development Zone Tianyuan Aquatic Products Co. , Ltd, Yantai 265618, China;Effects of a new immuno-growth promoting preparation on the growth and immunization of Scophthalmus maximus[A];[C];2005
2 JIANG Wei-ming,HAN Yao-quan,WU Wei-jun (Guangxi Fishery Institute,Nanning 530021);Development and application of high quality feed for shrimp raising[A];[C];2006
3 Guo Zhixun Li Zhuojia Lin Heizhao Ye le Feng Juan Yang Yingying Chen Yongqing (South China Sea Fisheries Institute,Chinese Academy of Fishery Science,Guangzhou 510300,China);Effects of the Bacilus sp. on growth and immunity of the white leg shrimp[A];[C];2003
【Citations】
Chinese Journal Full-text Database 5 Hits
1 Wang Lei; Li Guangyou; Mao Yuanxing; Zhang Haiyan (Institute of Oceanology, Academica Sinica, Qingdao 266071);EFFECT OF ORAL IMMUNO DRUGS FOR PREVENTION AND CONTROL OF DISEASES OF CULTURED PENAEUS CHINENSIS[J];Oceanologia Et Limnologia Sinica;1994-05
2 Jiang Xiaolu Ge Beilei( Department of Food Science and Technology, Ocean University of Qingdao, Qingdao,266003);The Immunological Function of Cordyceps militarisY3 Intra cellular and Extra cellular Polysaccharide[J];Journal of Ocean University of Qingdao;1998-02
3 Zhou Dinggang Zheng Weiming and Zhong Nina(Sichuan Agricultural University, Yaan 625000);RELATIONSHIP BETWEEN OOCYTE PHOSPHATASE ACTIVITY AND OVULATION AFTER INDUCED SPAWNING IN MONOPTERUS ALBUS[J];Acta Hydrobiologica Sinica;1993-02
4 Chen Jingchun and Shi Anjing(Department of Biology, Sichuan University, Chengdu, 610064);MALACOZOAN IMMUNOBIOLOGY RESEARCH: A REVIEW[J];ACTA HYDROBIOLOGICA SINICA;1996-01
5 Qi Fang-jun Jia Jing-fen Li Ji-sheng (Laboratory of Cell Biology,Biology Department,Lanzhou University,Lanzhou 730000);PRODUCTION OF PEROXIDASE INHIBITORS FROM PLANT CELLS INDUCED BY CELL WALL DIGESTION ENZYMES[J];Acta Biologiae Experimentalis Sinica;1993-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Yong et al (Fisheries College,Huazhong Agricultural University,Wuhan,Hubei 430070);Effects of Immuno-stimulants as a Feed Additive on Lysozyme Activity and Phagocytic Activity of Carassius Auratus[J];Journal of Anhui Agricultural Sciences;2007-01
2 WANG Yun et al (College of Life Sciences and Technology, Shanghai Fisheries University, Shanghai 200090);ffect of Five Species of Herbs on Nonspecific Immune Activity of Litopenaeus vannamei[J];Journal of Anhui Agricultural Sciences;2007-26
3 HE Ying et al (Guangxi Institute of Veterinary,Nanning,Guangxi 530001);Immunoregulation Effect of Sargassum Polysaccharides on White-leg Shrimp[J];Journal of Anhui Agricultural Sciences;2008-31
4 FENG Pei-yong et al(College of Life Science,Ludong University,Yantai,Shandong 264025);Breeding of High Cellulase Yielding Strain Induced by Chemical Mutagens[J];Journal of Anhui Agricultural Sciences;2008-33
5 DENG Hong-mei et al(College of Chemistry and Life Science,Maoming University,Maoming,Guangdong 525000);Effect of Anionic Surfactant SDS on Some Physiological Indexes of Tomato Seedling[J];Journal of Anhui Agricultural Sciences;2008-35
6 FENG Pei-yong et al(College of Life Science of Ludong University,Yantai,Shandong 264025);Selection of the High-yielding Cellulose-niger Strain[J];Journal of Anhui Agricultural Sciences;2008-36
7 HU Chan-na et al(Chuyang College,Zhejiang Normal University,Jinhua,Zhejiang 321004);Impact of Overweight Treatment on Rice Seed Germination and Physiology in Seedling[J];Journal of Anhui Agricultural Sciences;2009-03
8 LIU Wei-qiang et al(College of Animal Science,South China Agricultural University,Guangzhou,Guangdong 510642);Study on the Activity of Silkworm Lysozyme and Peroxidase[J];Journal of Anhui Agricultural Sciences;2009-05
9 HAN Li-ya et al (Life Science College of Ludong University,Yantai,Shandong 264025);Selection and Breeding of Trichoderma viride Strains with High Productivity on Cellulase by Compound Mutation[J];Journal of Anhui Agricultural Sciences;2009-09
10 GUO Zhi-hua et al(State Key Lab of Microbial Technology,Shandong University,Jinan,Shandong 250100);Screening of Bacillus Producing β-galactosidase and Optimization of Its Enzyme Production Conditions[J];Journal of Anhui Agricultural Sciences;2009-10
China Proceedings of conference Full-text Database 4 Hits
1 DAI Hui-ping FENG Bai-li GAO Xiao-li GAO Jin-feng WANG Peng-ke CHAI Yan (College of Agriculture,Northwest A & F University,Yangling,Shaanxi 712100,China);Root senescence and active oxygen metabolism in Panicum miliaceum L.[A];[C];2007
2 ;不同贮温下冬枣果实软化衰老特征研究[A];[C];2006
3 Guo Zhixun Li Zhuojia Lin Heizhao Ye le Feng Juan Yang Yingying Chen Yongqing (South China Sea Fisheries Institute,Chinese Academy of Fishery Science,Guangzhou 510300,China);Effects of the Bacilus sp. on growth and immunity of the white leg shrimp[A];[C];2003
4 ZHANG Tian-shu LI Kai ZHANG Li-li WANG Bin (School of Life Science,East China Normal University,Shanghai 200062,China);The Effect of the Artificial Diets on the Activity of Digestive Enzymes in Propylea japonica[A];[C];2008
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 PAN De-bin et al(Shenyang Agricultural University,Shenyang,Liaoning 110161);Effect of the Different Toxicity of Cadmium on Growth of Ostrinia furnacalis Guenée[J];Journal of Anhui Agricultural Sciences;2006-04
2 HE Yun et al(College of Animal Science,Henan Institute of Science and Technology,Xinxiang,Henan 453003);Study on Nutritional Value and Influencing Factors of Alfalfa[J];Journal of Anhui Agricultural Sciences;2007-11
3 WANG Yun et al (College of Life Sciences and Technology, Shanghai Fisheries University, Shanghai 200090);ffect of Five Species of Herbs on Nonspecific Immune Activity of Litopenaeus vannamei[J];Journal of Anhui Agricultural Sciences;2007-26
4 HUO Guang-ming et al(Nanjing Xiaozhuang College,Nanjing,Jiangsu 210017);Influence of Medicinal Fungal Substance on Enhancing the Immunity of Fugu obscurus[J];Journal of Anhui Agricultural Sciences;2008-03
5 YU Miao(College of Life Science,Henan Normal University,Xinxiang,Henan 453007);Research Progress in the Effect of Crowding Stress on Fish[J];Journal of Anhui Agricultural Sciences;2008-03
6 MA Yan et al(College of Life Sciences and Technology,Dalian Fisheries University,Dalian,Liaoning 116023);Study on the Quantity Dynamic Changes of Heterobacteria and Vibrios in Larvae Industrialized Culture System[J];Journal of Anhui Agricultural Sciences;2008-18
7 TIAN Juan et al (College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 200090);Effects of Chinese Herbal Formula on the Growth Performance,Muscle Composition and Serum Non-specific Immunity of Grass Carp[J];Journal of Anhui Agricultural Sciences;2008-28
8 YANG Wen-ping et al(College of Chemistry and Biology Engineering,Yancheng Institute of Technology,Yancheng,Jiangsu 224003);Effect of Colorant in Feed on Growth and Digestive Enzyme Activities of Pelteobagrus fulvidraco[J];Journal of Anhui Agricultural Sciences;2008-35
9 LV Fu et al(College of Chemical and Biological Engineering,Yancheng Institute of Technology,Yancheng,Jiangsu 224003);Study on the Toxicities of Ammonia-N to Perinereis aibuhitensis under Different Salinities[J];Journal of Anhui Agricultural Sciences;2009-07
10 HAN Bin et al(Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 201306);Effects of the Feed with Corn Gluten Meal Partial Replacement of Fish Meal on Anti-disease and Non-specific lmmunity of Litopenaeus vannamei[J];Journal of Anhui Agricultural Sciences;2009-08
China Proceedings of conference Full-text Database 9 Hits
1 HAO Lin - hua, CHEN Kao - shan, QU Jiang - bo, YANG Zhi The 1 st Institute of Oceanography, SOA; Key Lab of Marine Bioactivity Substance of State Oceanic Administration, Qingdao 266061, China; Life Science Institute, Shandong University, Jinan 250100, China; Yantai Development Zone Tianyuan Aquatic Products Co. , Ltd, Yantai 265618, China;Effects of a new immuno-growth promoting preparation on the growth and immunization of Scophthalmus maximus[A];[C];2005
2 JIANG Wei-ming,HAN Yao-quan,WU Wei-jun (Guangxi Fishery Institute,Nanning 530021);Development and application of high quality feed for shrimp raising[A];[C];2006
3 Wang Cheng-zhang~1 He Yun~(1,2) Gao Yong-ge~3 Shi Ying-hua~1 Li Ping~1 (1 College of Animal Husbandry and Veterinary,Henan Agricultural University,Zhengzhou 450002,China; 2 College of Animal Acience,Henan Institute of Science and Technology,Xinxiang 453003,China; 3 Forage Grass and Feedstuff Station of Animal Husbandry Bureau of Henan Province,Zhengzhou,450011,China);Effect of Alfalfa Meal on antioxidant function and lipid metabolism of Cyprinus Carpio haematopterus[A];[C];2008
4 He Yun~(1,2) Li Ming-feng~1 Wang Cheng-zhang~(1*) Gao Yong-ge~3 Zhu Wei-ran~3 Guo Jiang-zhe~1 Fan Wen-na~1 (1 College of Animal Husbandry and Veterinary,Henan Agricultural University,Zhengzhou 450002,China; 2 College of Animal Acience,Henan Institute of Science and Technology,Xinxiang 453003,China; 3 Forage Grass and Feedstuff Station of Animal Husbandry Bureau of Henan Province,Zhengzhou,450011,China);Effect of Alfalfa Meal on Growth Performance and Body Colour of Cyprinus Carpio haematopterus[A];[C];2008
5 Guo Zhixun Li Zhuojia Lin Heizhao Ye le Feng Juan Yang Yingying Chen Yongqing (South China Sea Fisheries Institute,Chinese Academy of Fishery Science,Guangzhou 510300,China);Effects of the Bacilus sp. on growth and immunity of the white leg shrimp[A];[C];2003
6 Fei Xing Wang Weimin (Guangdong Ocean University,Zhanjiang 524025,China);Applications in separation marine active polysaccharides by ultrafiltraion[A];Chinese Society of Agricultural Engineering[C];2007
7 ;药性菌质对提高暗纹东方鲀免疫力的影响[A];[C];2008
8 ZOU Jing-hu AI Tao-shan YU Yun-zhen;Effect of Nonspecific Immunity on Carassius auratus With Six Kinds of Chinese Medicines[A];[C];2008
9 ;Effect of Nonspecific Immunity on Carassius auratus With Six Kinds of Chinese Medicines[A];[C];2008
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 CHENG ZHEN-HENG LIANG ZI-CAIDepartment of Biology, Nankai University, Tianjin;STUDIES ON THE PHENOLOXIDASE IN THE HEMOLYMPH OF THE ASIAN CORNBORER OSTRINIA FURNACALIS[J];Acta Entomologica Sinica;1990-04
2 Zhou Hui-Ping; Jiang Xun-Tian; Wang Shu-Ru; Chen QiOng-Hua(Division of Biochemistry, China Pharmaceutical University, Manjing 210009);Effect of Polysaccharide from Enteromorpha prolifera on Lipemia,SOD Activity and LPO Content[J];;1995-02
3 Wang Xin Ma Guirong Zheng Baocan Tian Hao(Institute of Microbiology Shandong University, Jinan 250100);EFFECTS OF SL-PROBIOTIC PREPARATION ON THE BODY WEIGHT AND PHAGOCYTOSIS OF WHITE MICE[J];Acta Microbiologica Sinica;1995-06
4 Li Hua; Zhang Ling;Dong Li;Cao Jichao;Shi Qing;Zhang Yuanchao;(Dept .of Biolchemical Pharmaceutics ,shandong Medical Univetrsity ,Jinan 250012 );Antitumor activity and effect on immunocompetence of Plurotus os-treatus fungi glycopeptides components by oral preparation in Mice[J];PHARMACEUTICAL BIOTECHNOLOGY;1995-01
5 LI Xiong-Biao (Deparment of Biology, Peking University, Beijing 100871);Molecular Structure and Physiological Function of Enzymes in Plant Cell Wall[J];Plant Physiology Communications;1991-04
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