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《OCEANOLOGIA ET LIMNOLOGIA SINICA》 2000-03
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ACTIVITIES AND PROPERTIES OF SUPEROXIDE DISMUTASE AND CATALASE IN THE HAEMOLYMPH OF CHLAMYS FARRERI

SUN Hu-shan, LI Guang-you (First Institute of Oceanography, State Oceanic Administration, Qingdao, 266003)  
The activities and properties of superoxide dismutase (SOD, EC 1.15.1.1) and catalase (CAT, EC 1.1 1.1.6) in the haemocytes and serum of Chlamys farreri were studied from October 1997 to January 1998. The activities of SOD were measured with the method of autooxygenation of pyrogallol. The activities CAT were measured by the decrease in absorbance due to H_2O_2 consumption. The distinction of CuZn and Mn form of SOD, heat stabilities of SOD and CAT, effects of temperature on the activities of SOD and CAT were also studied. There are activities of SOD and CAT in both haemocytes and serum. SOD and CAT activities in haemocytes are 312.7U and 124.4U; and are 102.3U and 73.8U in serum.. 24.6% and 40.8% of SOD and CAT activities in serum are reduced after they were iced for 10 days. CuZn-SOD and Mn-SOD activities in haemocytes are 70.3% and 29.7% of the total SOD activities. CuZn-SOD is present but Mn-SOD is not detectable in serum. The heat stability of SOD is very high. SOD is highly active after the serum was beated in 80℃ for 30min. The heat stability of CAT is low. The optimum temperature for SOD and CAT in serum respectively appear about 50℃ and 45℃.
【Fund】: 国家攀登计划B资助项目!PD-B6-6-3
【CateGory Index】: Q55
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