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《Oceanologia Et Limnologia Sinica》 2008-01
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THE OPTIMAL CONDITION FOR GSH PRODUCTION FROM ANTARCTIC ICE MICROALGAE BERKELEYA RUTILANS GJ01

DING Yu~(1,2) MIAO Jin-Lai~3 LI Guang-You~3 JIAN Ji-Chang~(1,2) WU Zao-He~(1,2) (1.Fisheries College of Guangdong Ocean University,Zhanjiang,524025;2.Guangdong Provincial Key Lab of Pathogenic Biology and Epidemiology for Aquatic Economic Animals,Zhanjiang,524025;3.Key Lab of Marine Bioactive Substances,State Oceanic Administration,Qingdao,266061)  
GSH,as a key component of Asc-glutathione cycle,plays important roles in protecting tissues from toxicant damage and keeping cells in normal redox state,on which this paper studied.Berkeleya rutilans GJ01,one of so-called Antarctic ice microalgae,an extreme microorganism in the cryosphere,is one of major producers of primary production in marine ecology system of Antarctic polar region.They have become a promising resource of GSH.In this paper,effect of nutrition factor on GSH production level was studied for G J01 with spectrophotometer in searching for the optimization of synthesis condition for GSH.It was showed that 1.2g/L NaHCO_3 and 0.2mmol/L Ca~(2+) is ideal to the production of GSH. Vitamin B_(12) and biotin impacted slightly on GSH production.Orthogonal test revealed that the optimal condition for GSH production of GJ01 is 16h light/8h dark,1.6g/L NaHCO_3,0.4mmol/L Ca~(2+),and 10mmol/L Cys.The results should be in- formative to the application of GJ01 for GSH production.
【Fund】: 国家自然科学基金,40206022号、40406003号
【CateGory Index】: Q94-3
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