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《Oceanologia et Limnologia Sinica》 2011-06
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EFFECTS OF DIFFERENT REFINING METHODS ON THE MICRO-MORPHOLOGY,SPECTRA PROPERTIES AND METAL ELEMENTS OF MELANIN FROM SQUID(OMMASTREPHES BARTRAMI) INK

SONG Ru1,LI Ren-Wei2,YU Qun-Di1,DENG Shang-Gui1(1.School of Food Science and Pharmacy,Zhejiang Ocean University,Zhoushan,316000;2.Beijipin Aquactic Product Co.Ltd.of Zhejiang Province,Hangzhou,311215)  
In this study,pepsin hydrolysis,high speed centrifugation and acidic hydrolysis were used to refine the melanin from squid(Ommastrephes bartrami) ink,and the characteristics of surface morphology,optical spectra and metal elements of these refined squid melanin were investigated.Results of scanning electronic microscope(SEM) showed that acidic hydrolysis damaged the melanin’s original morphology.These refined squid melanin had maximum absorbance around 220nm.Fourier transform infra-red(FTIR) spectra revealed that pepsin hydrolysis and acidic hydrolysis cut off amino groups from squid melanin.However,the characteristic peak of indole group of melanin shifted from 1399.64cm-1 to 1307.88cm-1 after acidic hydrolysis.High speed centrifugation for squid melanin resulted in forming new peaks at 2925.02cm-1 and 2854.09cm-1,respectively.Moreover,the different refining methods lead to the effects of decrease or enrichment on the contents of metal elements in squid melanin.
【Fund】: 浙江省水产品加工产业创新团队项目 2011R09031-08号;; 浙江省自然科学基金项目 Y3110153号;; 浙江省优先主题重点项目 2011C13027-2号
【CateGory Index】: TS254.1
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