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《Oceanologia et Limnologia Sinica》 2012-04
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SUN Yan 1, 2 , LIU Fei 1, 2 , SONG Xiao-Ling 2 , MAI Kang-Sen 1 , LI Yu-Hong 2, 3 , HUANG Jie 2 (1. The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003; 2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071; 3. Shanghai Ocean University, Shanghai, 201306)  
This study was conducted to investigate the effects of Bacillus firmus and Vibrio alginolyticus on relative expression of non-specific immune related genes and antiviral ability of Litopenaeus vannamei. The relative expressions of non-specific immune related genes encoding prophenoloxidase (proPO), superoxide dismutase (SOD), lysozyme (LZM) were analyzed by quantitative real-time reverse transcription PCR (RT-PCR). The results show that the relative expressions of proPO, SOD, and LZM were obviously up-regulated in hemolymph of shrimp fed diets supplemented with the probiotics compared to the control during the immunization. In hemolymph, the relative expression of proPO, SOD, and LZM were significantly up-regulated 24h post WSSV challenge, and increased to the maximum levels at 48h, 96h, and 96h, respectively. The cumulative mortality results confirm that the diet containing probiotics can improve the ability of the shrimp anti-WSSV infection. Results suggest that B. firmus and V. alginolyticus are potential candidates for use as probiotics.
【Fund】: 公益性行业(农业)科研专项经费项目 201103034号;; 国家虾现代产业技术体系 CARS-47号
【CateGory Index】: S963
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Chinese Journal Full-text Database 4 Hits
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