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《Oceanologia et Limnologia Sinica》 2013-03
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CONSTRUCTION OF HAEMOLYMPH cDNA LIBRARY OF SCYLLA PARAMAMOSAIN INDUCED BY VIBRIO PARAHAEMOLYTICUS AND ANALYSIS OF EXPRESSED SEQUENCE TAGs

CAI Xiao-Hui, PENG Yin-Hui, ZHAO Peng, SONG Zhong-Kui, ZHANG Qin, HUANG Guo-Qiang (Guangxi Institute of Oceanology, Key Laboratory of Marine Biotechnology of Guangxi, Beihai, 536000)  
To obtain the potential immune-related genes and explore their function, the cDNA library of haemolymph from Scylla paramamosain induced by Vibrio parahaemolyticus was constructed by SMART technology. The results show that the titer of the library was 1.04×107CFU/mL, the library capacity reached 1.14×107CFU, the range of the cDNA inserts was 500—2000bp, and the recombinant rate was about 98%. A total of 300 clones of the library were randomly picked out and sequenced, and 270 raw sequences of the ESTs were processed and then assembled into 141 unigenes. Blast analysis show that 75 unigenes had significant homology to genes with known or putative protein function in non-redundant protein database of NCBI. There were 20 unigenes, 6.67% of total ESTs, related with immune defence functions, such as Kazal-like serine protease inhibitor-like protein, Kazal-type protease inhibitor, arasin-like protein, antilipopolysaccharide factor, antimicrobial peptide, metallothionein, ferritin, 70kD heat shock-like protein and so on. All above results confirm that constructing high-quality cDNA library was a reliable tool to screen out immune-related genes of S. paramamosain.
【Fund】: 广西自然科学基金资助项目 2010GXNSFA013079号 2012GXNSFAA053065号;; 广西科技攻关资助项目 1114012-11号;; 南海水产经济动物增养殖广东普通高校重点实验室开放课题 2011003号;; 国家海洋公益资助项目 201205028-4号
【CateGory Index】: S945
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