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《Marine Environmental Science》 2011-04
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Gene expression of oyster Crassostrea hongkongensis HSC70 of different size individuals and threshold induction concentration with Cu~(2+) challenge

ZHANG Zhan-hui,ZHANG Qi-zhong(Hydrobiology Institute of Jinan University,Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering Ministry of Education,Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institutes,Guangzhou 510632,China)  
The transcription changes of HSC70 gene of the oyster Crassostrea hongkongensis was quantified by means of real-time fluorescence quantitative RT-PCR method.The housekeeping gene β-actin was used as control gene.The different sizes of oysters which divided into two groups,one was 5~6 cm in length,and the other was 10~12 cm in length,were treated in 100 μg/L Cu2+ solution for 48 hours,and then the transcription changes of HSC70 gene was quantified in the gill,mantle,digestive gland and adductor muscle.The results showed that the expression levels in larger individuals of the oysters were significantly higher than smaller ones in mantle,gill and digestive gland,but not significant difference in the adductor muscle.When the similar size oysters were exposed to 0.01,0.1,1,10,50 and 100 μg/L Cu2+ solution for 6 h,2 d,4 d,8 d,20 d,35 d and 45 d,respectively,the threshold induction concentration of Cu2+ for HSC70 mRNA expression was in the range of 1~10 μg /L in the gill and mantle,and 0.1~1 μg /L in the digestive gland.The oyster HSC70 mRNA in the groups could induce,the HSC70 mRNA levels were significantly increased and reached the peak in gills and digestive gland for 2 days,and peaked for 4 days in mantle after Cu2+ exposure,and then dropped to control levels for 8 days or 20 days.In conclusion,the HSC70 gene response of C.hongkongensis,was sensitive to Cu2+ and could last for a longer time.Therefore,it could be used as a biomarker in response to Cu2+ pollution.
【Fund】: 国家自然科学基金项目(40576056;40976066)
【CateGory Index】: S917.4
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