Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Marine Environmental Science》 2011-04
Add to Favorite Get Latest Update

Gene expression of oyster Crassostrea hongkongensis HSC70 of different size individuals and threshold induction concentration with Cu~(2+) challenge

ZHANG Zhan-hui,ZHANG Qi-zhong(Hydrobiology Institute of Jinan University,Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering Ministry of Education,Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institutes,Guangzhou 510632,China)  
The transcription changes of HSC70 gene of the oyster Crassostrea hongkongensis was quantified by means of real-time fluorescence quantitative RT-PCR method.The housekeeping gene β-actin was used as control gene.The different sizes of oysters which divided into two groups,one was 5~6 cm in length,and the other was 10~12 cm in length,were treated in 100 μg/L Cu2+ solution for 48 hours,and then the transcription changes of HSC70 gene was quantified in the gill,mantle,digestive gland and adductor muscle.The results showed that the expression levels in larger individuals of the oysters were significantly higher than smaller ones in mantle,gill and digestive gland,but not significant difference in the adductor muscle.When the similar size oysters were exposed to 0.01,0.1,1,10,50 and 100 μg/L Cu2+ solution for 6 h,2 d,4 d,8 d,20 d,35 d and 45 d,respectively,the threshold induction concentration of Cu2+ for HSC70 mRNA expression was in the range of 1~10 μg /L in the gill and mantle,and 0.1~1 μg /L in the digestive gland.The oyster HSC70 mRNA in the groups could induce,the HSC70 mRNA levels were significantly increased and reached the peak in gills and digestive gland for 2 days,and peaked for 4 days in mantle after Cu2+ exposure,and then dropped to control levels for 8 days or 20 days.In conclusion,the HSC70 gene response of C.hongkongensis,was sensitive to Cu2+ and could last for a longer time.Therefore,it could be used as a biomarker in response to Cu2+ pollution.
【Fund】: 国家自然科学基金项目(40576056;40976066)
【CateGory Index】: S917.4
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 CHEN Jian-hua 1, 2, ZHANG Jun-bin 2, WANG Huai-min 1, ZHANG Xiao-jun 1, YAN Bin-lun 1( 1.Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005,C hina; 2.College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306,China);cDNA Cloning and mRNA Expression of HSC70in Oriental Weatherfish Misgurnus anguillicaudatus[J];Fisheries Science;2013-05
【Citations】
Chinese Journal Full-text Database 4 Hits
1 Dai Jiayin,Zheng Weiyun,Wang Shuhong (Environ.Sci.Res.Centre,Xiamen University,Xiamen 361005);Joints Toxicities of Heavy Metals and Pesticides to Pagrosomus major and Rhabdosargus sarba Larvae.[J];CHINESE JOURNAL OF ENVIROMENTAL SCIENCE;1997-05
2 Lü Jingcai 1,2, Zhao Yuanfeng 1,2, Wu Yichun1, Liu Changfa 1,2, Zhang Mingzhu1, Zhang Tianyang1(1.College of Life Science and Technology, Dalian Fisheries University, Dalian 116023, China;2.Key Laboratory of Mariculture and Biotechnology, Ministry of Agriculture, Dalian 116023, China);Accumulation of copper in the tissues of Chlamys farreri and its effects on the activity of superoxide dismutase in the seawater[J];Transactions of The Chinese Society of Agricultural Engineering;2005-05
3 Liu Chunying Zhang Zhengbin Liu Liansheng(Chemistry and Chemical Engineering college, Ocean University of Qingdao, Qingdao 266003, China);Study on Trace Metals in Coral Reef of Daya Bay, China[J];Journal of Ocean University of Qingdao;2001-04
4 HUO Wen mian 1, JI Wei dong 1, XU Kun can 1 (1 Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China);Dissolved Cu , Pb, Zn and Cd in the South China Sea surface waters[J];Acta Oceanologica Sinica;2002-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 CHEN Xin et al (Xiamen Ocean Vocational College,Xiamen,Fujian 361012);Study on Acute Toxicity of Five Common Drugs on Myrocyprinus asiatticus Juvenile[J];Journal of Anhui Agricultural Sciences;2008-22
2 SHI Chen et al(Research Center for Harmful Algae and Aquatic Environment,Life science and Technology College,Jinan University,Guangzhou,Guangdong 510632);The Acute Toxicity of Heavy Metals to Pseudodiaptomus Annandalei and the Effect on Ingestion[J];Journal of Anhui Agricultural Sciences;2011-12
3 ZHAN Ling-zhi, TIE Bo-qing, QING Pu-feng,MAO Xiao-qian, YUAN Min(College of Resources and Environment,Hunan Agriculture University, Changsha 410128, China );Toxic effect of cadmium and acetochlor on Spirodela Oligorrhiza[J];Journal of Safety and Environment;2005-03
4 LU Lei-da,JIN Ye-fei,SHI Wei-lin,SHANG Ke-dan,SUN Xiao,SUN Yong-bo(College of Life Sciences,Shaoxing Art and Technology University,Shaoxing 312000,China);Effects of Single and Complex Pollutions of Linear Alkylbenzene Sulfonates (LAS) and Cu~(2+) on Activities of SOD and CAT in Liver of Oriental Weatherfish Misgurnus anguillicaudatus[J];Fisheries Science;2007-12
5 WU Cui-qin (College of Environmental Science and Engineering,Guangzhou University,Guangzhou 510006,China);Estrogenic Effects of Diethylstilbestrol and Octylphenol on Juvenile Genuine Porgy Pagrosomus major[J];Fisheries Science;2008-12
6 LIU Hai-fang1,WANG Fan2(1.Energy and Eviromental Institue,Zhongyuan Technology College,Zhengzhou 450007,China,2.Life and Science Department,Luoyang Normal University,Luoyang 471022,China);Advanced Research on Pollution of Heavy Metals on Biomarkers in Aquatic Animals[J];Fisheries Science;2009-05
7 WANG Li-mei,BAO Peng-yun,DING Jun,CONG Yu-ting,WANG Yuan(Key Laboratory of Aquatic Biology in Province-level College in Liaoning,Dalian Ocean University,Dalian 116023,China);Acute Toxicity of Phoxim to Sea Cucumber Apostichopus japonicus Juveniles[J];Fisheries Science;2011-01
8 SHENG Lian-xi1,YU Wen-guang1,XU Jing-bo1,ZHENG Yong-chen2,WANG Yun-biao1(1.College of Urban and Environmental Sciences,Northeast Normal University,Changchun 130024,China; 2.The Second Hospital,Jilin University,Changchun 130022,China);Effects of temperature and copper on HSP70 expression in carp serum[J];Journal of Northeast Normal University(Natural Science Edition);2007-01
9 Yu\ Kefu ① \ Chen\ Tegu ① \ Lian\ Jiansheng ①\ Wang\ Zhaoding ① \ Liu\ Tungsheng ② (① South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301; ② Institute of Geology and Geophysics,Chinese Academy of Sci;ANNUAL CHANGES OF HEAVY METALS IN CORAL PLATYGYRA IN DAYA BAY AND THEIR MARINE ENVIRONMENT IMPLICATION[J];Quaternary Sciences;2002-03
10 LI Qi-lin1, 2, WEI Chao-fu1, ZENG Rong2, WANG Xian-jun3, XIE De-ti1 (1.School of Resource and Environment, Southwest University, Chongqing 400716; 2.Chongqing Monitoring Station of Agricultural Environmental Protection, Chongqing 400020; 3.Chongqing Chemical Industry Research Institute, Chongqing 400021);Characteristics of Copper in Different Aquacultures[J];Environmental Science & Technology;2007-11
【Co-references】
Chinese Journal Full-text Database 7 Hits
1 WAN Wen-ju1,2,3 WANG Ji-ting3, SHI Cun-bin1, WU Shu-qin1 (1.Pearl River Fisheries Research Institute,Chinese Academy of Fishery Science,Guangzhou 510380,China;2.Department of Basic Medicine,Taishan Medical University,Tai'an 271000,China;3.College of Animal Science & Technology,Shandong Agricultural University,Tai'an 271018,China);Gene expression of HSP70 in green swordtail Xiphophorus helleri exposed to Vibrio alginolyticus[J];Journal of Dalian Fisheries University;2007-05
2 CHENG Hanliang JI Nanjing PENG Yongxing SHEN Xin WU Chenchen XU Jianhe DONG Zhiguo(Jiangsu Key Laboratory of Marine Biotechnology,Huaihai Institute of Technology,Lianyungang 222005,China);Cloning and Expression Analysis of a Full-length cDNA Encoding Glucokinase from Pengze Crucian Carp (Carassius auratus var. Pengze)[J];Chinese Journal of Animal Nutrition;2011-07
3 YAO Dong-Rui1, BING Xu-Wen2, ZHU Ming1, BI Ke-Ran1, CHEN Li1, ZHANG Xiao-Jun1 (1. College of Ocean, Huaihai Institute of Technology, Lianyungang, 222005; 2. Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Science, Wuxi, 214081);MOLECULAR IDENTIFICATION AND DRUG RESISTANCE OF PATHOGENIC AEROMONAS SOBRIA ISOLATED FROM MISGURNUS ANGUILLICAUDATUS[J];Oceanologia Et Limnologia Sinica;2010-05
4 ZHANG Chong-ying1,2,XING Wei2,LI Tie-liang2,MA Zhi-hong2,JIANG Na2,LUO Lin2,LI Yun1(1.School of Animal Science and Technology,Southwest University,Chongqing 400715,China; 2.Beijing Fisheries Research Institute,Beijing 100068,China);Effects of heat stress on non-specific immune parameters and HSP70 gene expression in Taisho Sanke koi(Cyprinus carpio)[J];Journal of Fisheries of China;2012-03
5 SHAN Hong1,2,ZHOU Guo-qin1,ZHANG Qi-zhong2,3(1.Nanjing Fishery Science Institute,Nanjing 210036,China; 2.College of Life Science,Southwest China University,Chongqing 400715,China; 3.Institute of Hydrobiology,Jinan University,Guangzhou 510632,China);Cloning and sequence analysis of heat shock cognate 70(Hsc70) from Silirus meridionalis Chen[J];Journal of Shanghai Fisheries University;2007-04
6 ZHANG Juan1, ZHANG Qi-Zhong1,2, ZHANG Zhan-Hui1 and CUI Miao1(1. Hydrobiology Institute of Jinan University, Guangzhou 510632; 2. Key laboratory of Aquatic science of Chongqing, Key Laboratory Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing 400715);HSC70 GENE AND ITS TISSUE EXPRESSION ANALYSIS IN YELLOW CATFISH[J];Acta Hydrobiologica Sinica;2009-03
7 JIA Ai-Rong,WANG Chuan-Juan and ZHAGN Xiao-Hua (Department of Marine Biology,Ocean University of China,Qingdao 266003,China);CLONING AND EXPRESSION ANALYSIS OF A HSC70 GENE FROM TURBOT[J];Acta Hydrobiologica Sinica;2010-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Zhang Buchang Shang Guichun Yu Dapeng Mao Chunyan Li Baofang (School of Life Science Anhui University Hefei 230039);Study on Anti-fatigue of Oyster Oral Liquid[J];JOURNAL OF ANHUI UNIVERSITY(NATURAL SCIENCES);1999-01
2 YANG Li hua, FANG Zhan qiang, ZHENG Wen biao, WU Yu yuan, MA Guang zhi (College of Life Science, South China Normal University, Guangzhou 510631, China);EXPERIMENT WITH EFFECT OF CADMIUM ON ACTIVITY OF SUPEROXIDE DISMUTASE IN GILL AND LIVER TISSUE OF CRUCIAN[J];Journal of Safety and Environment;2003-03
3 NIU Chang-Ying ① JIANG Yong ① LEI Chao-Liang ① HU Cui ②(① Institute of Insect Resources, Huazhong Agricultural University Wuhan 430070; ②Institute of Applied Entomology, Zhejiang University, Huajiachi Campus Hangzhou 310029, China);Studies of Invertebrate Metallothionein[J];Chinese Journal of Zoology;2002-01
4 Lǔ Hai\|yan, ZENG Jiang\|ning, ZHOU Qing\|song, FU He\|fang, FENG Xu\|wen,WANG Zheng\|fang (Second Institute of Oceanography, SOA, Hangzhou 310012, China);Study on Hg, Cd, Pb and As in the shellfish along the coast of Zhejiang[J];Donghai Marine Science;2001-03
5 ZHOU Xian Qing ①② SUN Ru Yong ① NIU Cui Juan ① (①College of Life Science,Beijing Normal University,Beijing 100875,China);The Effects of Stress on Aquatic Animal's Growth,Behavior and Physiological Activity[J];Zoological Research;2001-02
6 WANG Yin\|qiu, ZHANG Ying\|mei, ZHAO Dong\|qin(School of Life Sciences, Lanzhou University, Lanzhou 730000,China);EFFECTS OF HEAVY METALS CADMIUM, LEAD ANDZINC ON THE SURVIVAL OF CARASSIUS AURATUS ANDMISGURNUS ANGUILLICAUDATUS[J];Journal of Gansu Sciences;2003-01
7 Zhuang Shuhong(Department of Biochemistry, Yantai University, Yantai, 264003)Liu Xuemei(Analyzing Center, Yantai University, Yantai, 264003);Chromium Concentration by 5 Species of Economic Marine Animals Sampled from Different Regions of Yantai Sea[J];JOURNAL OF OCEANOGRAPGY OF HUANGHAI &BOHAI SEAS;1996-02
8 Zhou Zhenhui Weng Chaolian (Shanghai Institute of Entomology, Chinese Academy of Sciences, Shanghai, 200025)Mo Hanhong (Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085);ACCUMULATION AND DESORPTION OF PESTICIDE N'-(2,4-DIMETHYLPHENYD)-N-METHYLFORMAMIDINE IN AQUATIC ECOSYSTEM[J];Environmental Chemistry;1997-02
9 CHEN Liang GUO Hong-yan SHEN Hong WANG Xiao-rong (State Key Laboratory of Pollution Control and Resource Reuse,School of the Environment,Nanjing University,Nanjing,210093);EFFECT OF TRACE LEAD ON THE ANTIOXIDANT SYSTEM OF FISH LIVER[J];Environmental Chemistry;2002-05
10 Dai Jiayin,Zheng Weiyun,Wang Shuhong (Environ.Sci.Res.Centre,Xiamen University,Xiamen 361005);Joints Toxicities of Heavy Metals and Pesticides to Pagrosomus major and Rhabdosargus sarba Larvae.[J];CHINESE JOURNAL OF ENVIROMENTAL SCIENCE;1997-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved