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Effects of five kinds of antibiotics stressed on chlorophyll fluorescence parameters of Isochrysis sp.CCMM5001

ZHENG Ming-gang1,ZHENG Li1,WANG Ling2,HAN Xiao-tian3,LI Hai-dong1,CUI Zhi-song1(1.The First Institute of Oceanography,Qingdao 266061,China;2.College of Chemical Engineering and Enviroment Qingdao University,Qingdao 266071,China;3.Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China)  
The different concentrations of antibiotics stressing on chlorophyll fluorescence parameters Fv/Fm(PSⅡ maximum solar energy conversion efficiency) of Isochrysis sp.were determined by Phyto-PAM.The results showed that there was large difference in the impact of different antibiotics on chlorophyll fluorescence parameters Fv/Fm of Isochrysis sp..Chloramphenicol,G-418 could significantly inhibit the maximum solar energy conversion efficiency of Isochrysis sp.The effect followed by the hygromycin.The effect was not observed by kanamycin and ampicilin for the maximum solar energy conversion efficency of Isochrysis sp.In the f/2 medium,stressed by chloramphenicol with concentration of 600 mg/L,Fv/Fm of Isochrysis sp.decreased by 97% on the third day.In f/2 medium,stressed by hygromycin with the concentrations of 1 200 mg/L and 500 mg/L G-418,Fv/Fm of Isochrysis sp.decreased by 52% and 98% on the 6th day.In f/2 medium diluted 5 times,stressed by hygromycin with the concentration of 1 200 mg/L and G-418 with the concentration of 500 mg/L,Fv/Fm of Isochrysis sp.decreased by 77% and 100% on 6th day.With increasing concentrations of Kanamycin and ampicillin stressed,Fv/Fm of Isochrysis sp.was not reduced,but increased.
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