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《Marine Sciences》 1995-05
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STUDIES OF SELECTIVE MARKER FOR KELP TRANSFORMATION

Wu Jianqiu,Wang Xihua, Song Qin and C. K. Tseng (Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071)  
In recent years, progress has been made in the gene transfer of seaweeds. Lack of selective marker made it difficult to acquire a stoblyexpressed transgenic kelp. Results of antibiotic sensitivity of young sporophytes of Laminaria japonica was reported in the present paper. It is proved that, Laminaria japonica is very sensitive to chloromycetin. LD60h50of young sporophytes (1. 5cm-2. 0cm in length) to of chloromycetin is 188. 8ug/ml and LD72h50is 154. 6ug/ml. LD60h50 of sporophytes from 3. 5cm to 4. 0cm in length is 406. 5ug/ ml and LD72h50 is 184. 6ug/ml. These results indicate that chloromycetin can be a selective marker and CAT gene will be a promising positive seloctive gene for kepl transformation.
【CateGory Index】: Q943.2
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