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Genetic diversity of ITS sequences in farmed and natural Rhopilema esculentum populations

SUN Guo-hua1,2,LIU Xiang-quan1,YANG Jian-min1,ZHANG Xi-jia1,LIU Ai-ying1,TAN Fu-yi2(1.Marine Fisheries Research Institute of Shandong Provience,Yantai 264006,China;2.Shandong Homey Aquatic Development Co.LTD,Weihai 264305,China)  
For the purpose of investigating genetic diverstiy in wild and cultured populitions of Rhopilema escu-lentum,ITS regions of 24 individuals within Yantai and Jiangsu wild populitions and Weihai cultured population were amplified and analyzed.The whole sequences were between 1 080~1 096 bp,including ITS1-5.8S-ITS2.The result showed that 26 variable nucleotide positions were detected and four nucleotide sites had 29 insertion-deletion positions.Indexes of Pi and K among three populations were 2.179~2.750 and 0.002 02~0.002 54,respectively.In-dexes of K,genetic distance and fixation indices(Fst) between populations were 2.797~3.031,0.002 30~0.002 81 and 0.032 50~0.730 8,respectively.The indexs of genetic diversity among and between populations were closed and genetic distances between populations were small,which indicated that the genetic structure of populations were smilar and there were no distinct genetic differentiation between populations.
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