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《Progress in Fishery Sciences》 2010-03
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Progress and prospect of colloidal gold-based immunochromatographic lateral flow assay and its application in aquaculture

WANG Wei-fang1 LI Qing-mei2 GUO Jun-qing2 LEI Ji-lin1(1Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,266071)(2Henan Key Laboratory for Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002)  
Immunoassays are demonstrated to be simple,rapid,and cost effective.For the past decades,more and more techniques are being developed which combine the advantages of approaches in highly powerful analytical tools.The convenience and speed of the test have been achieved by a novel concept of colloidal gold-based immunochromatographic assay,which depends on the transport of a color-labeled reactant to its binding partner immobilized on the surfaces of the membrane.This new technique has been proved to be a well-established and appropriate technique for a variety of point-of-care and field-use applications,which was widely used as a convenient test for human being and terrestrial animal diseases in nowadays.In this paper,progress on the study of this new technique has been summarized in detail,which focuses on its principle,application and development.Based on these,its practicability in aquatic animal is also introduced.It would provide a new point of view on the innovation of simple and fast detection of disease in aquaculture.
【Fund】: 农业部公益性行业科研重大专项(NYHYZX-07-046);; 国家鲆鲽类产业技术体系(NYCYTX-50);; 山东省博士后创新项目专项资金(200902008)共同资助
【CateGory Index】: S941
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