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《Progress in Fishery Sciences》 2013-02
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Antimicrobial activity of hydrolysates from Ruditapes philippinarum

LIU Qi1 XIE Sha1,2 ZHAO Ling1 CAO Rong1 WAN Hui-yi1,2 WEI Yu-xi2 (1Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071) (2Biological Department, Qingdao University, 266071)  
The antibacterial activities of the hydrolysates from Ruditapes philippinarum, prepared by pepsin,acid protease, bromelin, trypsin and papain, were compared. Orthogonal experiments were employed to optimize the preparation process of the hydrolysate with the greatest antibacterial activity, and molecular weight distribution of the optimal hydrolysate was determined. The hydrolysate of pepsin showed antibacterial activity against Staphylococcus aureus,Escherichia coli, Bacillus subtills, Saccharomyces cerevisiae and Vibrio parahaemolyticus. The optimal hydrolysis conditions for pepsin were as follows: 35℃, pH 2.0, reaction time 2 hours,dosage of protease 3 000U/g, and solid to liquid ratio 1∶4. The molecular weight of the pepsin hydrolysate were mainly below 5 000.
【Fund】: 国家科技支撑计划(2012BAD28B05)资助
【CateGory Index】: S917.4
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