Isolation and Identification of Aeromonas salmonicida Associated with Furunculosis in Cultured Sablefish(Anoplopoma fimbria)
WANG Xiaoran;CHEN Siqing;MO Zhaolan;LI Guiyang;LIU Changlin;WANG Youting;LI Jie;HUANG Jie;College of Marine Life Sciences, Ocean University of China;Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology;Yantai Haiyi Seeds Co., Ltd;
Sablefish(Anoplopoma fimbria), also known as black cod, is a sleek, black-skinned and valuable cold water benthic fish species that lives in the north Pacific Ocean. They possess high content of unsaturated fatty acids. Sablefish have been cultured in British Columbia of Canada, and in recent years, they were introduced into the aquaculture industry in Shandong Province in north China. In this study, we reported the observation of furunculosis found in cultured sablefish in China. AF-1, a strain of bacterial pathogen was isolated from the diseased sablefish. Sablefish infected with this bacterium showed typical symptoms of furunculosis such as subcutaneous hemorrhage in the abdomen and the fin, abscess, ulcer and liver congestion. AF-1 was confirmed to be the pathogen of sablefish furunculosis through the artificial injection experiment, in which the injected fish showed the same symptoms. A series of methods including biochemical and biophysical tests and phylogenetic analysis of 16 S r RNA and gyr B genes were used to identify the pathogen. The API ID32 E results showed that AF-1 belonged to Aeromonas sp. The 16 S r RNA and gyr B genes were amplified and sequenced, and the submission numbers were KU359246 and KU362567. Phylogenetic analysis of gene sequences showed that AF-1 was Aeromonas salmonicida. The chemotherapeutant sensitivity test showed that AF-1 was resistant to 13 antibiotics such as penicillin, amoxicillin and cephalothin, but was sensitive to another 16 antibiotics such as fumequine and florfenicol. According to the antibiotics sensitivity test result, florfenicol was added into diets to control furunculosis in sablefish. Our study indicated that A. salmonicida could infect cultured sablefish in our country, and provided information on the disease control in cultured sablefish.
【Fund】： 国家自然科学基金(31402346);; 中国水产科学研究院黄海水产研究所基本科研业务费(20603022016005 20603022017008);; 山东省自主创新及成果转化专项(2014ZZCX06205);; 青岛海洋科学与技术国家实验室鳌山科技创新计划(2015ASKJ02)共同资助~~
【CateGory Index】： S943
【CateGory Index】： S943