Isolation and Classification of Exophthalmic Disease-Inducing Pathogenic Bacterium from Centropristis striata
CHEN Jianguo;CHEN Chao;LI Yanlu;SUN Shuguang;SHAO Yanxiang;ZHANG Tingting;LIU Li;SUN Tao;College of Fisheries and Life Science, Shanghai Ocean University;Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;Dalian Ocean University;Yantai Tianyuan Fisheries Corporation;
A strain of bacterial pathogen was isolated from eyeballs of cultured Centropristis striatawith the symptoms such as white chaotic, hyperaemia and paraplasm in the eyeballs. The strain grew quickly in the TCBS culture medium, and the colonies were protruding, yellow, and tackiness. The strain named CJG01 was Gram-negative, bacillus and monotricha. The intramuscular injection experiment revealed that CJG01 was virulent to C. striata with LD_(50) value of 2.67×10~5 CFU/ml. CJG01 could cause symptoms including larval exophthalmos, eyeball fall off, muscle ulceration, and fishbone exposure. Anatomical observation showed liver inflammation, splenic organ enlargement, kidney inflammation and yellow liquid in the intestine. The results of API 20 NE rapid identification and related physiological and biochemical experiments showed that the growth temperature for CJG01 was 28℃–37℃, and the optimum temperature was 28℃. CJG01 could grow in TSB culture medium with 0–5% salinity. It was sensitive to vibrio inhibitors O/129, was oxidase and ornithine decarboxylase positive, and was V-P negative. CJG01 could assimilate mannitol, maltose and malic acid, but not glucose, arabinose, mannose, decanoic acid, acetic acid, citric acid, benzene, etc. The strain shared similarities with Vibrio harveyi in biochemical and biophysical properties. Analysis of the 16 SrDNA sequence and the phylogenetic tree suggested that CJG01 was highly similar to V. harveyi. Based on the results above, CJG01 was identified as V. harveyi. The chemotherapyeutant sensitivity test showed that CJG01 was insensitive to ampicillin, cephalosporins ammonia benzyl, cephalosporins, norfloxacin, penicillin, azithromycin, polymyxin B, etc. It was moderately sensitive to cefazolin, streptomycin, erythromycin, clarithromycin, etc. Novobiocin, neomycin, cefoperazone, ceftriaxone, gentamicin, cefoperazone, kanamycin, ofloxacin, ciprofloxacin, fleroxacin, furazolidone, rifampicin, ciprofloxacin, tetracycline, chloroamphenicol and minocycline could effectively inhibit the growth of CJG01.
【Fund】： 国际合作项目(2012FDA30360);; 东盟项目“中国-东盟海水养殖技术联合研究与推广中心”共同资助~~
【CateGory Index】： S943
【CateGory Index】： S943