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Study about CSF activity induced by sepia in mice

XIE Guang lin,HE Shen (Department of basic medical,China medical university,ShenYang 110001)  
To study the inducing effect of sepia on the CSF (colony stimulating factor) level of mice.The BALB/c mice be poured into sepia through mouth for five days,then obtaining the marrow cell,culturing it in Methyl cellulose M 20 which contains CSF and lasted time of CSA (colony stimulating activity) for assay by method colony forming,The results indicated that sepia could promote colony forming of marrow cell of mice and as compared with control it has a significant differences ( P 0.01),and CSA may last 6 days,after 8 days CSA gradully dropped.
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