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《Chinese Journal of Marine Drugs》 2005-02
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Effects of chitosan on proliferation and differentiation of human periodontal ligament cells

MAO zhao~ 1, YANG Jian~ 2,MAO Xi~ 3,SU Han~ 1 (1.Department of Odontology , Nanjing General Hospital of PLA , Nanjing 210002 , China;2.Department of pharmacology,Nanjing Medical University, Nanjing 210029 China;3.Bisic Medical college of Dongnan University,Nanjing 210093,China)  
Objective To observe effects of chitosan on proliferation and differentiation of human periodontal ligament cells( PDLC). Methods The human periodontal ligament cells were isolated and cultured.The effects of chitosan on proliferative ability of human PDLCs were evaluated with MTT method, and alkaline phosphatase activity and the secretion of osteocalcin of human PDLCs were measured with spectrophotometric assay and radioimmunological method. Results Compared with control group, the proliferative ability of human PDLCs in Chi(0.05g·L~ -1) group and Chi(0.1g·L~ -1) group at 3,5,7 day was considerably increased (P0.05), alkaline phosphatase activity of human PDLCs in all chitosan groups was significantly enhanced (P0.05) and the secretion of osteocalcin of human PDLCs in Chi(0.1g·L~ -1) group was obviously promoted (P0.05). Conclusion These results suggested that the chitosan could promote the proliferation and differentiation of human PDLCs.
【Fund】: 江苏省科技厅高技术研究资助项目(BG2001033);; 中国人民解放军南京军区十五医药卫生基金资助项目(No.02MB003)
【CateGory Index】: R780.2
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