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《Chinese Journal of Marine Drugs》 2006-02
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Determination of hypoxanthine and xanthine in Syngnathus by HPLC

CUI Xiao-bing,LI Wei,ZHANG Ke-wei,CAI Hao,WU Hao(Research Center on New and Marine Drugs of Nanjing University of TCM,Nanjing 210029,China)  
Objective To establish a HPLC method for determination of hypoxanthine and xanthine in Syngnathus.Methods A Lichrosper C_(18) Column was used.The mobile phase was methanol-0.1%HAc.Walvelength was 254nm.Results The linear range of hypoxanthine was within 5.91~94.56μg·mL~(-1),r=0.9999,sample recovery rate was 99.22%,RSD=(1.25)%.The linear range of Xanthine was within 2.04~32.64μg·mL~(-1),r=0.9998,sample recovery rate was 98.05%,RSD=1.21%.Conclusion This method has good repeatability and flexibility.It can be used for quality control in production of Syngnathus.
【CateGory Index】: R284
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