Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Marine Drugs》 2006-03
Add to Favorite Get Latest Update

Extraction, purification and immunological activity assay of a polysaccharide from eggs of sea urchin Strongylocentrotus nudus

LIU Chun-hui, Xi Tao , Lin Qin-xiong, Gao Yi, Xing Ying-ying(School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China)  
Objective To extract and purify a polysaccharide SEP from eggs of sea urchin Strongylocentrotus nudus. and to determine its purity, molecular weight and immunological activity in vitro. Methods The orthogonal design was employed to obtain the best possible combination of the critical parameters for polysaccharide extraction. By ultrafiltration, DE-AE Sepharose Fast Flow anion-exchange chromatography and Sephacryl S-400 gel filtration chromatography, the deproteinated crude polysaccharide was purified. The homogeneity of SEP was proved by HPLC, polyacrylamide gel electrophoresis and paper chromatography. Its molecular weight was determined by HPGPC in reference to standVd T-series Dextran. Lymphocyte proliferation assay was made to investigate the immuno-modulating activity of SEP. Results and Conclusion The results indicated SEP was a homogeneous polysaccharide. Its molecular weight was about 1950KD. SEP increased remarkably spleen lymphocyte proliferation. The homogeneous polysaccharide SEP showed significantly immunological activity in vitro.
【CateGory Index】: R284
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 2 Hits
1 HE Yun-mian;CHEN Yu-xing;LIU Chun-hui;XI Tao;SHEN Zi-long;YAO Quan-sheng 1.School of life Science and Technology,China Pharmaceutical University,Nanjing 210009,China;2.The Drug Safety Evaluation Center of Jiangsu Province,Nanjing 210009,China;Isolation,purification and immunological activity assay of polysaccharide from Arca subcrenata Lischke[J];Chinese Journal of Marine Drugs;2007-02
2 ZHOU Hong1,LIN Cui-wu1,ZHU Ding-ji1,HUANG Ke-jian2,HUANG Guan1,LU Jie1 (1.College of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004,Guangxi,China;2.Institute of Forensic Science of Guangxi Provincial Public Security Department,Nanning 530012,Guangxi,China);Study on Ultrasonic Extraction and Analysis of Fatty Acids in Gonads of Temnopleurus Toreumaticus(Leske)[J];Fine Chemicals;2010-03
Chinese Journal Full-text Database 10 Hits
1 CHENG He Li,ZHAO Xin Min 1 (Dept of Pharmacy , the First Affiliated Hospital of Anhui Medical University,Hefei 230022; 1Liansheng Science and Trading Co.Ltd,Hefei 230022);The eperimental research of anti-inflammation effect of jasminoidin[J];Anhui Medical and Pharmaceutical Journal;2004-03
2 SUN Xu-qun, ZHAO Xin-ming , YANG Xu (Dept. of Pharmacy,The First Hospital Affiliated to Anhui Medicine University,Anhui Hefei 230022,China);Experimental Research on the Cholagogic Action of Gargenoside[J];Journal of Anhui Traditional Chinese Medical College;2004-05
3 Li Dan Rong1,Hou Hua Xin2,Zhang Wei1,Li Li1 1.Clinic Experiment Center,Guangxi Cancer Institute,Nanning,Guangxi, 530021,P.R.China 2.Department of Chemistry,Guangxi Medical University,Nanning, Guangxi,530021,P.R.China;Effects of Wogonin on Inducing Apoptosis of Human Ovarian Cancer A2780 Cells and Telomerase Activity[J];Chinese Journal of Cancer;2003-08
4 ZHU Yue-xiong, SHI Liang, CAO Guang-li (Life Science College,Suzhou University, Suzhou 215006, China);Decoloration and Deproteinization of Polysaccharose from Wild Ganoderma applanatum[J];Amino Acids & Biotic Resources;2005-03
5 MIAO Yue-qiu~1,MEI Qiu-hong~1,GU Gong-ping~2,SHEN Qi~1,LU Chang-mei~1,WU Guo-rong~1(1.College of Life Sciences,Nanjing Normal University,Nanjing 210097;2.Nanjing Institute for Comprehensive Utilization of Wild Plant,Nanjing 210042);Optimization for the enzyme hydrolysis of fenugreek neutral heteropolysaccharide and the effects of its products on reproduction of Bifidobacterium adolescentis[J];Journal of Anhui Agricultural University;2006-02
6 Chan Meiying,Cheng Huijuan,Le Hongxia (Department of Microbiology,Anhui College of Tradiltional Chinese Medicine,Hefei);SUSCEPTIBILITY TESTING OF GARDNERELLA VAGINALIS TO TRADITIONAL CHINESE MEDICINES[J];ACTA ACADEMIAE MEDICINAE BENGBU;1995-04
7 Chu Lisheng, Zhang Shihong, Yu Guoliang, et al Zhejiang College of TCM,Hangzhou (310053);Effect of Baicalin on Permanent Focal Cerebral Ischemia in Mice[J];Journal of Zhejiang College of Tcm;2005-06
8 Zou Jieming et al ( Cooperated Limited Company of Guilin Sanjin Pharmaceutical Industry );Experimental Research of Effectual Composition of Chinese Herbs and Drugs Separated by Hyperfiltration Technique[J];China Journal of Traditional Chinese Medicine and Pharmacy;2003-02
10 LI Su-ting,MEI Li-xin,YANG He-mei,et al(Chengde Medical College,Hebei Chengde 067000,China);EFFECTS OF SSTF ON EXPRESSION OF TGF-β_1 IN RATS WITH LIVER FIBROSIS[J];Journal of Chengde Medical College;2005-04
【Secondary References】
Chinese Journal Full-text Database 6 Hits
1 LI Yue et al(Shandong University at Weihai,Weihai,Shandong 264209);Study on Marine Medical Mollusk in Cheniers and Wetland Nature Reserve of Wudi[J];Journal of Anhui Agricultural Sciences;2008-17
2 ZHAO Yan-jing1,HU Hong2,WANG Ying3 1.Jiangsu Key Laboratory of Marine Biotechnology,Huaihai Institute of Technology,Lianyungang 222005,China;2.Lianyungang Higher Vccational Technical College of Traditional Chinese Medicine,Lianyungang 222005,China;3.China Pharmaceutical University,Nanjing 210009,China);Extraction of Polysaccharides from Razor Clam Sinonovacula constricta and Their Antioxidant Activity[J];Fisheries Science;2010-02
3 WANG Li1,HE Yun-mian2,YAO Quan-sheng3* (1.Nanjing University of Technology,Nanjing,Jiangsu,210009 P.R.China;2.China Pharmaceutical University,Nanjing,Jiangsu,210009 P.R.China;3.Jiangsu Center for Safety Evaluation of Drugs,Nanjing,Jiangsu,210009 P.R.China);Studies on immunomodulation of polysaccharide from Arca subcremata Lischke[J];West China Journal of Pharmaceutical Sciences;2009-04
4 WANG Yong1,YANG Jing1,SUN Xun2,LI Rong-gui1(1.Department of Biology,Qingdao Medical College,Qingdao 266021,China;2.Department of Science & Research,Qingdao University,Qingdao 266071,China);Analysis of antioxidative activity of extract from Arca subcrenata Lischke[J];Chinese Journal of Marine Drugs;2008-03
5 ZHAO Heng-qiang1,2,CHEN Jun-hui2,WANG Hong2,ZHANG Dao-lai2,WANG Xiao-ru2,LI Guo-qiang2(1.College of Chemistry and Chemical Engineering,Ocean University of China,Qingdao 266100,China; 2.Qingdao Key Lab. on Analytical Technology Development and Standardization of Chinese Medicines,First Institute Oceanography of SOA,Qingdao 266061,China; 3.College of Medicine and Pharmacy,Ocean University of China,Qingdao 266100,China);Study on the HPLC fingerprint of sea urchin Hemicentrotus pulcherrimus[J];Chinese Journal of Marine Drugs;2009-03
6 ZHOU Hong1,LIN Cui-wu1,ZHU Ding-ji1,HUANG Ke-jian2,HUANG Guan1,LU Jie1 (1.College of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004,Guangxi,China;2.Institute of Forensic Science of Guangxi Provincial Public Security Department,Nanning 530012,Guangxi,China);Study on Ultrasonic Extraction and Analysis of Fatty Acids in Gonads of Temnopleurus Toreumaticus(Leske)[J];Fine Chemicals;2010-03
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 WU Wu-tong CHEN Qiong-hua (Nanjing College of Pharmacy) XU Bi-ru WANG Sai-zhen HHEN Jin-tu (Sanming Fungi Research Institute in Fujian, Sanming);ISOLATION AND ANALYSIS OF POLYSACCHARIDE FROM THE SPORES OF TREMELLA FUCIFORMIS[J];Mycosystema;1982-02
2 Lü Yan 1 , Chen Hao 1, Wang Shuijuan 1, Fei Xiugeng 2, Zhang Weiyun 1 (1.Department of ment Biology, Nanjing University, Nanjing, 210093, China; 2.Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266073, China);Isolation, Purification and Structural Analysis of Polysaccharides from Porphyra haitanensis (Ⅱ)[J];Journal of Naijing University (Natural Sciences);2001-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved