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《Chinese Journal of Marine Drugs》 2007-04
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Optimization of fermentation medium and characteristics of antibacterial substances from marine actinomycete JMC06001

XIAO Huai-dong,ZHANG Xiao-rong,LIU Zhu-xiang,PENG De-jiao,ZHANG Li,CHEN Yi-guang,TAN Yu,HUANG Wen-yue (Key Laboratory of Bio-resources﹠Environmental Ecology,Institute of Ecology,Jishou University,Jishou 416000,China)  
Objective To optimize the fermentation medium of the marine actinomycete strain JMC06001,which exhibited strong antibacterial activity,and to study the characteristics of antibacterial substances produced by strain JMC06001.Methods The fermentation medium was optimized by using single-factor experiment and orthogonal test.The effect of ultraviolet light,temperature and pH on the stability of antibacterial substances was investigated,and the hydrophobic nature of the antibacterial substance was detected.Results and Conclusion The optimum composition of the fermentation medium was as follows: glucose 15 g·L-1,peptone 10 g·L-1,soybean flour 14 g·L-1,NaCl 30 g·L-1 CaCO3 1 g·L-1,broth A of compound salts 20 mL·L-1,broth B of compound salts 1 mL·L-1, and the optimum original pH value of the fermentation medium was 7.0.The antibacterial substance exhibited better thermal stability and light stability.It only exhibited antibacterial activity at pH 5~7,whereas it was inactive under strong acidity condition or high alkalic condition.The antibacterial substance was well soluble in organic solvents,such as chloroform,ethyl acetate,normal butanol,etc.
【Fund】: 福特基金项目(07JDPHE031 07JDPHE053);; 吉首大学人才引进基金项目;; 湖南省教育厅科学研究项目(C05168 00C191)
【CateGory Index】: R282
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