Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Marine Drugs》 2011-02
Add to Favorite Get Latest Update

Extraction,purification and physicochemical properties analyses of Mytilus edulis Linnaeus polysaccharides

YIN Xiu-hong,ZHAO Xia,ZHANG Zi-heng,YAN Min,YU Guang-li*(Key Laboratory of Marine Drugs,Ministry of Education,Shandong Provincial KeyLaboratory of Glycoscience and Glycoengineering,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China)  
Objective To extract,isolate and purify polysaccharides from Mytilus edulis Linnaeus and analyze physicochemical properties of these polysaccharides.Methods Polysaccharides were extracted by cold distilled water and then by combined enzymolysis(papain and trypsin).The contents of total sugar,protein,uronic acid,glycosaminoglycans and sulfate were determined.Mytilus edulis Linnaeus polysaccharides were further purified using Sephacryl S-300 and Q Sepharose 4 Fast Flow chromatography.Monosaccharide composition,purity and relative molecular mass of components after purification were analyzed.Results After purification,eight components with good solubility were obtained from the polysaccharides extracted by cold water and by enzymolysis,respectively.Among these polysaccharides,monosaccharide composition of the water-extracted components LTS1-A was simple,only including Glc.While other components had complex monosaccharide composition,and mostly including Man,GlcN,Gal and Fuc.All the components showed a single peak on High Performance Gel Permeation Chromatography.Relative molecular masses of these components were in the range of 3.0 ~ 40 kD.Conclusion Polysaccharides with different physicochemical properties were extracted by cold distilled water and by combined enzymolysis.Components with high purity were obtained by Two-step Purification.Our data will provide references for the further researches on structure and activities of Mytilus edulis Linnaeus polysaccharides.
【Fund】: 国家自然科学基金(31070724);; 国家海洋局公益性行业专项(201005024)
【CateGory Index】: R284.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved