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《Chinese Journal of Marine Drugs》 2013-04
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Extraction,separation and structural characterization of polysaccharides from Neverita didyma

XING Xiao-xu,ZHAO Xia*,LI Da-jing,YU Guang-li,YIN Xiu-hong(Key Laboratory of Marine Drugs,Ministry of Education,Shandong Provincial Key laboratory of Glycoscience and Glycoengineering,School of Medicine and Pharmacy, Ocean University of China,Qingdao 266003,China)  
Objective To extract and purify polysaccharides from Neverita didyma,and investigate their chemical properties and structural features.Methods Neverita didyma polysaccharides were extracted by distilled water and alkali solution sequentially,and purified by DEAE Sepharose FF anion-exchange and Sepharcryl S-300 gel-permeation chromatography.The contents of total sugar,protein,uronic acid,sulfate and amino sugar were determined by various chemical methods,and monosaccharide compositions were analyzed by high performance liquid chromatography.The structure of polysaccharides was characterized by methylation analysis,GC/MS,FT-IR,ESI-MS and NMR techniques.Results No uronic acid,sulfate and amino sugar were found in the water-extracted polysaccharide(BYL-S) and its monosaccharide composition was simple,which only included Glc.Four water-soluble fractions were isolated by further purification from BYL-S.While BYL-J had complex monosaccharide composition,including Glc,Man,GlcN,GalN,Gal and Fuc,and their molar ratio was Glc∶ Man∶ GlcN∶GalN∶Gal: Fuc=78.9∶1.7∶3.4∶2.2∶5.2∶5.6.After further isolation,three fractions were obtained from BYL-J.The structure of BYL-S2 was characterized to be composed of α-(1→4)-D-glucopyranose,with little β-(1→3,4) and β-(1→3) branched units.Conclusion Seven polysaccharides were separated from Neverita didyma and the structure of a water-soluble polysaccharide(BYL-S2) was determined.These results will provide basis for further research on relationships of structure and activity of Neverita didyma polysaccharides.
【Fund】: 国家海洋局公益性行业专项(201005024);; 国家“十二五”科技支撑计划(2013BAB01B02);; 青岛市科技局项目(11-2-2-1-hy)资助
【CateGory Index】: S917.4
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【Citations】
Chinese Journal Full-text Database 5 Hits
1 YANG Hong-li et al(College of Food and Pharmaceutical Science,Zhejiang Ocean University,Zhoushan,Zhejiang 316000);Extraction and Content Determination of Polysaccharides from Neverita didyma[J];Journal of Anhui Agricultural Sciences;2012-13
2 ZHENG Huai ping GAO Jian(Department of Marine Engineering , Engineering College of Yancheng, Yancheng, 224003);SEASONAL CHANGES OF PROTEIN AND FAT OF Nevertia didyma (Rding)[J];Marine Sciences;2002-04
3 YIN Xiu-hong,ZHAO Xia,ZHANG Zi-heng,YAN Min,YU Guang-li*(Key Laboratory of Marine Drugs,Ministry of Education,Shandong Provincial KeyLaboratory of Glycoscience and Glycoengineering,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China);Extraction,purification and physicochemical properties analyses of Mytilus edulis Linnaeus polysaccharides[J];Chinese Journal of Marine Drugs;2011-02
4 HUANG Fang-fang,YANG Yong-fang,DING Guo-fang School of Food Science and Pharmacy,Zhejiang Ocean University,Zhoushan 316004,China;Advances in the study of antitumor activities of the marine mollusks extracts[J];Journal of China Pharmaceutical University;2009-03
5 FAN Qiao-yun,LI Chao-pin,WANG Ke-xia(School of Medicine,Anhui University of Science & Technology,Huainan,232001,Anhui,China);New advances in the study of the biological activity of polysaccharides from marine animals[J];Journal of Pathogen Biology;2012-11
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Qing SUN Zhen-Xing(Life Science College,Ludong University,Yantai 264025,China);Experimental Observation on the Early Development of Neverita didyma[J];Chinese Journal of Zoology;2008-05
2 HUANG Xiao-Chun~1, LIU Hui-hui~1, SU Xiu-rong~1, LIU Bao-zhong~2, LI Tai-wu~1(1. Faculty of Life Science and Technology, Ningbo University, Ningbo 315211, China; 2 Institute of Oceanology, Chinese Academy of Science, Qingda 266071, China);Fatty Acid Levels in 7 Economic Shellfishes[J];Fisheries Science;2005-08
3 CHEN Xiao-gang1,HU Hui-bin1,LIU Ai-wen1,CHEN Xin1,RONG Hai-wu2,SUN Hui-li3(1.Department of Chemistry and Chemical Engineering,Foshan University,Foshan 528000,China; 2.Department of Information Sciences and Mathematics,Foshan University,Foshan 528000,China; 3.Institute of Oceanology,Chinese Academy of Science,Guangzhou 510301,China);Study on the oxidation resistance and reducibility of enzymatic hydrolysis and cooking liquor of Mytilus edulis in vitro[J];Guangdong Agricultural Sciences;2011-21
4 LIU Yuling JI Guoli Taian Municipal Testing Center for Food and Drugs,Taian 271000,China;Extraction and content determination of polysaccharides from Fructus Mori[J];China Medicine and Pharmacy;2012-18
5 LI Li et al ( Zhejiang Provincial Key Engineering Technology Research Center of Marine Biomedical Products,School of Food Science and Pharmacy,Zhejiang Ocean University,Zhoushan,Zhejiang 316004);Study on the Anticancer Activity of the Ruditapes philippinarum Hydrolysate on the Lung Cancer H1299 cells[J];Journal of Anhui Agricultural Sciences;2013-14
6 LI Lian-jun et al(School of Food Science and Pharmacy of Zhejiang Ocean University,Zhejiang Provincial Key Engineering Technology Research Center of Marine Biomedical Products,Zhoushan,Zhejiang 316004);Study on Antiproliferative Activity of Ruditapes philippinarum Hydrolysate on Human Prostate Cancer DU-145 Cell[J];Journal of Anhui Agricultural Sciences;2013-15
7 ZHANG Yong-pu, YING Xue-ping, JIA Shou-ju(School of Life and Environmental Sciences, Wenzhou Normal College, Wenzhou 325027,China);Yearlong changes of the meat condition index, water contents, and biochemical compositions of blood clam (Tegillarca granosa)[J];Henan Science;2004-01
8 SUN Zhen-Xing,CHANG Lin-Rui,XU Jian-Peng(College of Life Science,Ludong University,Yantai,264025);EFFECT OF PHENOTYPIC MORPHOMETRIC TRAIT ON BODY WEIGHT AND SOFT-TISSUE WEIGHT OF NEVERITA DIDYMA[J];Oceanologia Et Limnologia Sinica;2010-04
9 ZHANG Liang,GENG Yue,TU Wen-li,ZHANG Jing-jing(Key Laboratory of Animal Resistant Biology,College of Life Science,Shandong Normal University,Jinan 250014,China);Determination of taurine in Nevertia didyma(Roding) and Asterias rollestoni Bell by HPLC[J];Marine Sciences;2009-04
10 LUAN Jie1,XIN Tian2,CHU ZHi-yong1,WANG Jia-chun1,ZHANG Shu1,SHEN Yue1(1.Naval Medical Research Institute,Shanghai 200433,China;2.Liaoning University of Traditional Chinese Medicine,Dalian 116600,China);Immunomodulation of Mytihus edulis Linnaeus polysaccharides in mice[J];Chinese Journal of Marine Drugs;2010-04
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Fu-xi et al(Liaoning Medical University,Jinzhou,Liaoning 121001);Immune Regulation of Cartilage Polysaccharides in Stingray[J];Journal of Anhui Agricultural Sciences;2010-06
2 YU NAN,LIU SAI,HAN JIAN-JIAN,et al(Department of Pharmacology,Qingdao University Medical College,Qingdao 266021,China);THE DEPRESSIVE EFFECT OF GLYCOSAMINOGLYCAN FROM SCALLOP ON TYPE-Ⅰ HERPES SIMPLEX VIRUS[J];Acta Academiae Medicinae Qingdao Universitatis;2008-02
3 LI Jiang-bin , HUANG Di-nan(Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang 524023, China);A Review: Pharmaceutical Values of Mussel[J];Fisheries Science;2004-11
4 YIN Hong-ling,MA Yuan,WANG Lu,SUN Yu-mei,YU Yun-hai,ZHU Bei-wei(College of Biotechnology & Food Technology,Dalian Institute of Light Industry,Dalian 116034,China);The Extraction of and Scavenging Hydroxyl Free Radicals by Scallop Viscus Polysaccharide[J];Fisheries Science;2007-05
5 LI Jiang-bin1,ZHANG Hai-feng2,LAI Yin-xuan3,HUANG Di-nan1(1.Biochemistry and Molecular Biology Institute of Guangdong Medical College,Zhanjiang 524023,China;2.Preclinical Medicine College of Zhengzhou University,Zhengzhou 450002,China;3.Affiliated Hospital of Guangdong Medical College,Zhanjiang 524023,China);Inhibition of Influenza Virus Reproduction in Chicken Embryo by Polysaccharides from Mussel(Perna viridis)[J];Fisheries Science;2007-06
6 LI Dong-mei,TAN Feng-zhi,YANG Jing-feng,YIN Hong-ling,LI Tao,ZHU Bei-wei(College of Biology and Food Technology,Dalian Polytechnic University,Dalian 116034,China);Isolation,Purification and Identification of Polysaccharides from Abalone Viscera[J];Fisheries Science;2007-09
7 LIN Hou-Wen , GAO Yong, LIU Gao-Lin , WANG Jie-Jun, ZHANG Chun( Department of Pharmacy, Changzheng Hospital t Second Military Medical University, Shanghai 200003, China; Department of Oncology, Changzheng Hospital);Studies on in vitro antitumor activities of a new sterol HEO from sea hare[J];Academic Journal of Second Military Medical University;2004-05
8 WANG Jun,XU Hong-li,ZHANG Jian-peng,LIU Jun-hua,FENG Wei-hua~(*),JIAO Bing-hua~(*) (Department of Biochemistry and Molecular Biology,College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433,China);Preparation,identification and immunological activities of polysaccharides extracted from Mytilus coruscus[J];Academic Journal of Second Military Medical University;2006-01
9 XU Hong-li~(1),GUO Ting-ting~(2),GUO Yi-feng~(1),ZHANG Jian-peng~(1),FENG Wei-hua~(1*),JIAO Bing-hua~(1*)(1.Department of Biochemistry and Molecular Biology,College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433,China;2.Central Laboratory,Aqua-life Science and Technology College,Shanghai Fisheries University,Shanghai 200090);Isolation and purification of water-soluble polysaccharide MP-Ⅰ from Mytilus coruscus and study on its in vitro anti-tumor activity[J];Academic Journal of Second Military Medical University;2006-09
10 LIU Na1,ZHU Bei-wei1,SUN Li-ming1,LIU Jun-rong2 1.School of Food Science and Technology,Dalian Polytechnic University,Dalian 116034,China;2.School of Food Science and Technology,Dalian Ocean University,Dalian 116023,China;Antioxidant activity of polysaccharide extracted from abalone gonad[J];Journal of Dalian Polytechnic University;2011-03
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