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Two-cuff-one-stent Technique for Rat Orthotopic Pulmonary Transplantation

ZHANG Xin,QIAO Chenhui,ZHI Xingyi,et alDepartment of Cardiothoracic Surgery,First Hospital Affiliated to Zhengzhou University,Zhengzhou 450052,China  
Objective To improve the former cuff techniques,and discuss the two-cuff-one-stent rat lung orthotopic transplantation model.Methods Using two-cuff-one-stent technique(to anastomose pulmonary arteries and veins in traditional cuff technique,and anastomose bronchi in stent technique),to establish the orthotopic lung transplantation model in rats.Results After 10 days of operation,the checks of left lungs were almost normal, and there was no significant difference of the blood gas analysis PO_2(132.4±12.5)mm Hg vs(126.3±11.6)mm Hg,P0.05,PCO_2(46.0±4.7)mm Hg vs(49.2±3.8)mm Hg,P0.05 before and after transplantation from the left pulmonary vein.Conclusion This model is much more simplified,reliable and economical to use on studying lung preservation transplantation.
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