Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Nanhua University(Medical Edition)》 2006-03
Add to Favorite Get Latest Update

The Identification and Screening of the High Invasive Metastatic Strain of Mice Histiocytosarcoma L-Ⅱ

LEI Zhan-ping,ZHU Jian-si,WANG Cheng-kun,et al(Institute of Cancer Research,Nanhua University,Hengyang,Hunan 421001,China)  
objective To screen the high invasive metastatic strain of mice histiocytosarcoma L-Ⅱ by constant subculturing of the cells from the pulmonary metastasis in vivo.Methods Histiocytosarcoma L-Ⅱ cells were subcutaneously injected in the flank of mice,then the cells in pulmonary metastasis were isolated and injected in another mouse by the same methods.After several subculturing,the high invasive metastatic strain of mice histiocytosarcoma L-Ⅱ were established.The isolated cells were analyzed by morphlogy,immunohistochemistry,karyotype,flow cytometry,cell growth curve,mean life span with cancer,and the rate of lung metastasis.Results We obtained the 7th generation now.There were no differences between the 1st and the 7th generation in morphology observation under light microscope.Chromatosome analysis showed they were all hypotetraploid,and their modal number were about 66-70.The cell cycles between the 1st and the 7th were: stage G_1:70.1/50.8;stage S:15.9/21.6;stage G_1: 14.0/21.6;respectively.Their DNA ploid were hypotetraploid.Mean life span with cancer was 22/16.33 day,respectively(P0.01).Immunohistochemistry showed that nm23 was weakly positive in the 1st generation and negative in the 7th generation.Lung metastasis was 25%(3/12)/66.7%(8/12),respectively;P=0.099.Conclusion We screened a high invasive metastatic strain of mice histiocytosarcoma L-Ⅱ,thus provided an ideal experimental model for the invasion and metastasis of tumor.
【CateGory Index】: R738
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 2 Hits
1 Gan Runliang,et al (Cancer Research Institute,Hengyang Medical College,Hunan );Establishment of a Model of Tumor Invasion and Metastasis with Transplantable Murine Histiocytic Sarcoma[J];ACTA LABORATORIUM ANIMALIS SCIENTIA SINICA;1997-02
2 LI Yan, TANG Zhaoyou, YE Shenglong, LIU Yinkun, CHEN Jie, XUE Qiong, HUANG Xiaowu, CHEN Jun, BAO Weihua, YANG Jiong, GAO Dongmei. Liver Cancer Institute and Zhong Shan Hospital of Fudan University, Shanghai, 200032, China;Establishment of human hepatocellular carcinoma cell line with spontaneous pulmonary metastasis through in vivo selection[J];National Medical Journal of China;2002-09
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Liu Hu,Ye Sheng -Long ,Li Wen-Chang ,Li Yan,Liu Yin-Kun,Qin Lun-Xiu,Qiu Shuang -Jian,Sun Rui-Xia,Chen Jie,Tang Zhao-YouLiver Cancer Institute /Zhongshan Hospital,Fudan University,Shanghai,200032,P.R.China;Quantitative Analysis of Expression of TEY1 Gene in Liver Cancer Cells with Different Metastatic Potential[J];Chinese Journal of Cancer;2003-06
2 PENG Zhihong~1, TANG Bo~2, YANG Jianmin~1, SI Suihai~1, CHEN Wensheng~1, FANG Dianchun~1, LUO Yuanhui~1 (~1Gastroenterology Research Center, ~2Department of General Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Effects of metastasis suppressor gene KAI1 on adhesion forces of MHCC97-H hepatocellular carcinoma cells with high metastatic potential[J];Acta Academiae Medicinae Militaris Tertiae;2005-23
3 CHEN Liangdong,LI Yan,ZHENG Fang,LI PengchengDept.of Oncology,Zhongnan Hospital of Wuhan University & Cancer Center of Wuhan University,Wuhan 430071,China;Study on the Intraperitoneal Chemotherapy with Hydroxycamptothecin for the Treatment of Peritoneal Carcinomatosis in Mice[J];Medical Journal of Wuhan University;2008-06
4 WU Xu-dong1,CHEN Wei-chang1,ZHU Shu-yang2,YE Jian-xin1 1.Department of Gastroenterology,First affiliated Hospital of Soochow University,Suzhou 215006,P.R.China2.Department of Pediatrics,North Courtyard of First People's Hospital of Chenzhou City,Chenzhou 423000,P.R.China;Effect of RNA interference targeting at peroxisome proliferator-activated receptor gamma gene on angiogenesis of hepatocellular carcinoma in nude mice[J];Chinese Journal of Cancer Prevention and Treatment;2009-07
5 Xue JF, Xie HB 1Department of General Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China; 2Department of General Surgery, Fifth People's Hospital of Zhengzhou, Zhengzhou 450003, Henan Province, China;Influence of thrombospondin-1 on the growth and adhesion of human hepatoma carcinoma cell line HCCLM3[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2007-43
6 Ming-Feng Zhao, Department of Surgical Pathology, Huashan Hospital, Fudan University, Shanghai 200040, China Lu Wang, Yan Zhao, Zhao-You Tang, Liver Cancer Institute and Zhongshan Hospital, Fudan University, Shanghai 200032, China. Zu-De Xu, Department of Pathology, Fudan University, Shanghai 200032, China;Effects of IFN-αon expression of angiogenic factors in highly metastatic human hepatocellular carcinoma cell line HCCLM3 cells[J];World Chinese Journal of Digestology;2004-06
7 WENG Yong-qiang, QIU Shuang-jian, TANG Zhao-you, YE Sheng-long, LIU Yin-kun, FAN Jia, SUN Rui-xia, ZHAO Yan. Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China;Changes in the immune function of dendritic cells (DC) derived from HBV-related hepatocellular carcinoma (HCC) patients' peripheral blood monocytes (PBMC) pulsed with tumor antigen[J];Chinese Journal of Hepatology;2005-05
8 CHENG Wen-wu, SONG Ming-zhi, XUE Qiong, SHEN Ye-hua. Cancer Hospital, Fudan University, Shanghai 200032, China;Effects of strengthening and regulating spleen-qi recipe on nude mice with metastatic human hepatocellular carcinoma[J];Chinese Journal of Hepatology;2005-06
9 LIU Hu,YE Sheng-long,XUE Qiong,et al.Liver Cancer Institute & Zhong Shan Hospital,Fudan University;Key Laboratory of Carcinogenesis and Cancer Invasion,Ministry of Education,Shanghai 200032,China;The establishment of a spontaneous metastatic model of rat liver cancer in nude mice[J];Chinese Journal of Experimental Surgery;2004-01
10 LI YAN*, TANG ZHAO-YOU, WANG LU,YAO ZHI, LU RONG, XUE QIONG, GAO DONG-MEI, CHEN JUN.*LIVER CANCER INSTITUTE AND ZHONGSHAN HOSPITAL OF FUDAN UNIVERSITY, SHANGHAI 200032, CHINA;STUDY ON THE INHIBITORY EFFECT OF SMALL TRIPEPTIDE CMS024 ON TUMOR GROWTH AND METASTASIS IN NUDE MICE MODEL OF HUMAN HEPATOCELLULAR CARCINOMA METASTASIS[J];National Medical Journal of China;2004-08
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LI Li 1,ZHANG Sheng2,LIN Hua2,LIN Jian-yin1* 1.Department of Molecular Medicine,Fujian Medical University,Fuzhou,350004,P.R.China2.Department of Pathology,The First Affiliated Hospital,Fujian Medical University,Fuzhou 350005,P.R.China;Relationship of Expression Unbalance of Matrix Metalloproteinase and Tissue Inhibitor of Metalloproteinase to Invasiveness and Metastasis in Gastric Carcinomas[J];Chinese Journal of Cancer;2002-03
2 CHEN Shan-yi, WANG Xiu-qing, LI Xian-ming, CHEN Yi-xin, WU Chao-quanDepartment of Radiation Oncology, Second Affiliated Hospital, Medical College of Jinan University, Shenzhen 518020, China;Expressions of P16 and nm23-H_1 in nasopharyngeal cacinoma and their clinical significance[J];Journal of First Military Medical University;2005-03
3 Bas takoti Baburam,SUO Tao,TONG Sai xiong,Dept.of General Surgery,ZhongshanHoSpital,University,Shanghai 200032);EXPRESSION OF E-CADHERIN PROTEIN IN GALLBLADDER CARCINOMA AND ITS CLINICAL SIGNIFICANC[J];Journal of Hepatobiliary Surgery;2004-03
4 DANG Yamei, LI Min, WANG Jinsui Gansu Provincial People’s Hospital, the Third Clinical Medical College, Lanzhou Medical College, Lanzhou, 730000, China;Recent Advances in nm 23 Genes[J];Foreign Medical Sciences;2004-06
5 LI Wen+1, WEN Yu-ming+2, WANG Li-hong+1, YANG Zhan+3,ZHANG Rui-lin+4. (1.Dept. of Otolaryngology, West China Hospital, Sichuan University, Chengdu 610041, China;2.Dept. of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu 610041, China;3.Dept. of Oral and Maxillofacial Surgery, College of Stomatology, Kunming Medical College, Kunming 650031, China;4.Dept. of Otolaryngology, The Second Renmin Hospital of Chengdu City, Chengdu 610017, China);In vitro and in vivo Study on Proliferation of Adnoid Cystic Carcinoma Cell Lines after nm23-h1 Introduction[J];West China Journal of Stomatology;2004-02
6 YUAN Yin, WANG Yi-fei, ZHANG Mei-ying, XIONG Sheng, LI Jiu-xiang, HU Hong-mei, LIU Qiu-ying, WU Zhi-cong (Jinan Biomedicine Research & Development Basement, Jinan University, Guangzhou 510632, China);Relationship between nm23-H1 gene expression and proliferation of HL-60 cells[J];Journal of Jinan University(Natural Science & Medicine Edition);2004-04
7 YE Lihong, QIN Xiaoran, ZHANG Xiaodong, WANG Honghui, JIANG Siming, WANG Changye, WU Jinghui, ZHU Huifang, ZHAO Shixuan, CAI Bing( College of Life Sciences, Nankai University, Tianjin 300071 China;Institute for Hematology, Peking Union Medical University, Tianjin 300020 China;Tianjin Institute for Biomedicinal Research, Tianjin 300384, China);SCREENING OF HUMAN METASTATIC HEPATOMASUB-CLONE BY SCID MICE AND EATABLISHMENT OFTHE M-H7402 SUB-CELL LINE[J];Acta Scientiarun Naturaltium Universitatis Nankaiensis;2004-03
8 XI Yan,DUAN Rui sheng,SONG Wei,et al.Department of Immunology,Shandong Provincial Qianfoshan Hospital,Jinan 250014,China;Immunological Molecule and gene research progress in Gastric Carcinoma Metastasis[J];Journal of Qilu Oncology;2002-06
9 Wu Junzheng, Situ Zhengqiang, Liu Bin, et al.College of Stomatology, Fuorth Military Medical University Xi'an 710032;Selection of highly metastatic cells of human salivary gland mucoepidermoid carcinoma by in situ transplantation of Mc3 cells in nude mice[J];Journal of Practical Stomatology;2001-06
10 Ting Liu, Xin-Yun Wang, Wen-Jing Song, Cong-Zhong Zhu, Yan Li, Department of Pathology, Tianjin Medical University, Tianjin 300070, China;Incidence of gastric malignant tumors during the past 20 years in Tianjin[J];World Chinese Journal of Digestology;2004-01
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 Zhou Xiaoge; YanQinghan; Zhang Xiaoping ( Department of Pathology,Beijng Hospital,Beijing 100730);Expression of Epstein-Barr virus encoded small RNA-1 and latent membrane protein-1 in midline T-cell lymphomas[J];CHINESE JOURNAL OF PATHOLOGY;1995-02
2 LI Yan TANG Zhaoyou YE Shenglong,Liver Cancer Institute,Zhongshan Hospital,Fudan University,Shanghai 20032,P.R.China;Isolation and establishment of human hepatocellular carcinoma cell clones with various metastatic potentiais[J];Chinese Journal of Hepatobiliary Surgery;2001-11
3 Zhou Kexiang;Wu Bingquan;Zheng Jie, et al.(Department of Pathology, BeijingMedical University, Beijing 100083);Effects of TIMP-1 cDNA transfection on biologicalbehaviors of PG cells[J];NATIONAL MEDICAL JOURNAL OF CHINA;1994-07
4 ZHANG Siwei, LI Liandi, LU Fengzhu, et al. National Office of Cancer Prevention and Treatment, Beijing 100021;Mortality of primary liver cancer in China from 1990 through 1992[J];CHINESE JOURNAL OF ONCOLOGY;1999-04
5 Tang Zhao-you et al;HUMAN HEPATOCELLULAR CARCINOMA (HCC) MODEL IN NUDE MICE-ESTABLISHMENT OF HCC TISSUE MODEL AND ITS APPLICATION IN EXPERIMENT RESEARCH[J];Tumor;1986-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved