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《Journal of Nanhua University(Medical Edition)》 2008-03
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Apoptosis of Nasopharyngeal Carcinoma Cells Line CNE1 Induced by Diallyl Disulfide

YANG Ke,TANG Hai-lin,SU Qi,et al (Medical College of Nanhua University,Hengyang,Hunan 421001,China)  
Objective To study the effect of diallyl disulfide on CNE1. Methods The growth inhibition of CNE1 cells was measured by MTT.The effect of DADS on induction of apoptosis of CNE1 cells was studied by light microscopy and flow cytometry,respectively.Bcl-2,Bax were determined using immunohistochemical.Western blot analysis was performed to detect the expression of Caspase-3 protein. Results MTT assay showed that DADS inhibited the growth of CNE1 cells.Partial cells presented characteristic morphological changes of apoptosis.Flow cytometry analysis showed that treating CNE1 cells with DADS increased the percentage of apoptosis cells.Immunohistochemistry revealed that the Bax expression was increased while Bcl-2 expressed was decreased.Western Blot analysis showed that Caspase-3 expression were increased.Conclusion DADS could significantly induce apoptosis of CNE1,Apoptosis of tumor cells is closely associated with down-regulation of the ratio of bcl-2/bax.then activation of Caspase-3.
【Fund】: 湖南省卫生厅科研基金(B2007098)
【CateGory Index】: R739.63
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