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《Journal of Medical Science in Central South China》 2012-01
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The Effect of PHCD on the Expression of NF-κB in Acute Lung Injury Rabbits

ZENG Jun-fa,XIAO Hong,GUI Pei-gen(Department of Intensive Care Unit,the Second Affiliated Hosptial,University of South China, Hengyang,Hunan 421001,China)  
Objective To observe the effect and protective mechanism of PHCD on the expression of NF-κB in acute lung injury(ALI) rabbits induced by lipopolysaccharide(LPS).Method 20 New Zealand rabbits were randomly divided into 4 groups:control group,model group,low and high PHCD-dose group.The low and high PHCD groups were intraperitoneally administrated by PHCD(0.2mg/kg and 2mg/kg,respectively) 30min before LPS injection,then the differences of lung morphology were observed.Immunohistochemistry and Western blot were used to detect the activation of NF-κB.Result Histomorphology showed that alveolar septum became thick and infiltrated with large amount of inflammation cells,and Bronchial mucosa occasionally fell off.After the treatment of PHCD,the inflammatory response was reduced in dose-dependent manner.Immunohistochemistry showed that only a few scattered NF-κB positive cells in the bronchial epithelial and interstitial lung in the control group,while in the model group,the NF-κB-positive cells increased significantly in the mucosa,pulmonary interstitial and alveolar and vascular endothelial.After PHCD treated,NF-κB positive cells were less than the model group,and the high dose group was more obvious.Western blot showed that in the lung tissue of model group,the p65 level of NF-κB was significantly higher than the negative control group,and p65 were significantly decreased after PHCD treatment.Conclusion PHCD could effectively reduce inflammation reaction in the ALI rabbits,which may be associated with the downregulation of NF-κB activity.
【CateGory Index】: R965
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