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《Journal of Zhejiang University(Sciences Edition)》 2001-06
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Study on heat shock protein and thermotolerant mechanism of S.cerevisiae

JIN Cheng-tao, ZENG Yun-zhong, WU Xue-chang, ZHU Xu-fen (College of Life Science, Zhejiang University, Hangzhou 310028, China)  
The differences of protein synthesis and the survivors in high temperature between the thermotolerant S.cerevisiae stain HU-TY-1 and its primary stain LK have been compared by SDS-PAGE(sodium dodecanoyl sulphate-polyarcy lamide gel electrophoresis)and counting colonies. The results show that both the stains can synthesize HSP72 and HSP84 in heat shock response, and HU-TY-1 can synthesize these HSPs weakly at normal temperature. This suggests that the high thermotolerance of strain HU-TY-1 is relative tightly to the constitutive synthesis of HSP70 and HSP84.
【Fund】: 浙江省自然科学基金资助项目 (395 177)
【CateGory Index】: Q935
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