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《Journal of Zhejiang University(Science Edition)》 2008-05
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Sequence analysis of mitochondrial fragment COⅡ gene of H. labeo and H. maculates from Oujiang River

LV Yao-ping1,2, HU Ze-hui3, YE Li-ping1, YAO Zi-liang4(1. College of Chemistry and Life Sciences, Lishui University, Lishui 323000, Zhejiang Province, China; 2. College of Animal Science, Zhejiang University, Hangzhou 310029, China; 3. Marine Fisheries Research Institute of Zhejiang Province, Zhoushan 316100, Zhejiang Province, China; 4. Lishui Fishery Technology Promotion Station of Zhejiang Province, Lishui 323000, Zhejiang province, China)  
Partial sequences of the mitochondrial cytochrome oxidase unit Ⅱ(COⅡ)gene of 2 species of Hemibarbus proliferated by Polymerase Chain Reaction(PCR), and other four species of Hemibarbus from GenBank were analyzed to determine the utility of this regions in identifying phylogeographic and phylogenetic structure. Nucleotide sequences of 600 bp in length were obtained from COⅡ gene for 20 Hemibarbus specimens' representing populations of H. labeo and H. maculates from Oujiang River, with A+T contents from 56.9% to 57.8%, which were obviously higher than G+C contents. Analysis of sequence variation at the COⅡ gene across five species revealed 116 variable sites, in which 46 were parsim-informative sites. Analysis of sequence variation across two H. labeo and H. maculatus populations revealed 11 variable sites, in both series. The base substitution was transition in H. labeo and the ratio of transition (Si) and transversion (Sv) was 8/3 in H. maculatus. The phylogenetic trees of Hemibarbus were reconstructed by using COⅡ gene sequences as marker, and with Paracanthobrama guichenoti Bleeker as outgroup. Tree topologies indicated that haplotypeⅠof H.maculatus(Zhejiang) and H. maculates(Jiangsu) formed a monophyletic group with the haplotype Ⅱ of H. maculatus(Zhejiang). H. labeo from South Korea and H. barbus were sister group, and then formed a clade with H. maculatus from China. H. longirostris and H. mylodon were formed a single clade, which suggested that the relationship between them were closer. The genetic distances between H. labeo from South Korea, and H. maculatus(Jiangsu) H. barbus and haplotypeⅠof H. maculatus(Zhejiang) were 0.0084 in both cases. Combined together morphologic characteristics, it was infered that H. labeo from South Korea and H. barbus should be treated as synonym of H. maculates from China.
【Fund】: 浙江省自然科学基金资助项目(No.Y307445);; 浙江省科技厅新苗人才计划资助项目(No.2007R40G2260026);; 浙江省教育厅资助项目(No.20070552);; 浙江省丽水市科技局资助项目(No.2007kjh2005)
【CateGory Index】: S917.4
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