The Establishment And Application of PCR to Diagnosis Porcine Circovirus Type 2
Cao Shengbo Chen Huanchun Xiao Shaobo He Qigai Xu Yindi Liu Junfa (Huazhong Agricultural University, Wuhan 430070,China)
According to the published genome sequences of porcine circovirus type 2 (PCV?2) a pair of specialprimers was designed to detect PCV?2 in samples from some farms by PCR. The PCR product was extracted and linked to pMD 18?T vector and then was colned into E.coli .DH5α.The recombination plasmid was identified by digestion with different enzyme restrictions, PCR and sequencing analysis. The homology of nucleotide sequence between the PCR product and other PCV?2 strains was more than 90%. Within forty three samples, twelve positive samples were found by PCR. It showed that PCV?2 infection is popular in our country.
【CateGory Index】： S858.28