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《Journal of Huazhong Agricultural》 2003-02
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Establishment of Efficient Regeneration System from Leaf Pieces and Internodal Segments of 9 Chrysanthemum Cultivars

Jiang Xiwang 1,2) Liu Guofeng 1) Bao Manzhu 1) ( 1) College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China; 2) College of Medicine and Life Sciences, Jianghan University, Wuhan 430056, China)  
This paper deals with efficient regeneration system from leaf pieces and internodal segments of 9 chrysanthemum cultivars with six differentiation media and three rooting media.The effect of genotype,combinations of plant growth regulators, concentrations of silver nitrate on differentiation was investigated .The results showed that optimized differentiation media were:'Japanese Yellow':6 BA 0.5 mg/L+NAA 0.1 mg/L or 6 BA 2.0 mg/L+NAA 0.5 mg/L;'9718'?'RH3'?'Winter Purple'?'Winter Yellow':6 BA 2.0 mg/L+NAA 0.5 mg/L;'Japanese White':6 BA 0.5 mg/L+NAA 0.1 mg/L;'Winter Red'?'Hang Bai Ju':6 BA 1.0 mg/L+NAA 0.1 mg/L;'Wu Jiu Ju':6 BA 2.0 mg/L+NAA 0.1 mg/L. Optimized rooting media were: 1/2MS+0.1~0.2 mg/L NAA?or 0.2~0.4 mg/L IAA?or 0.4~0.6 mg/L IBA?Silver nitrate restrained shoot regeneration.
【Fund】: 武汉市科学技术局技术计划项目 ( 2 0 0 2 5 0 0 7170 7)
【CateGory Index】: S682.11
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