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《Journal of Huazhong Agricultural》 2004-01
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Phylogenetic Relationships of Pleurotus species Based on rDNA PCR-RFLP

Ma Fuying Luo Jing Luo Xinchang ( State Key Laboratory of Agricultural Microbiology and Institute of Applied Mycology, Huazhong Agricultural University, Wuhan 430070, China)  
28S rDNA 5' half, ITS and IGS1 region of eighteen Pleurotus taxa, together with Agaricus bis-porus, Lentinula edodes and Hohenbuehelia serotina were amplified using PCR and then digested with seven restriction endonucleases. A single uniform 1. 46 kb product resulted from PCR amplification of 28S rDNA 5" half of four genera. Amplification of ITS resulted in a single 680 bp product for Pleurotus, 700 bp for H. serotina, 720 bp for L. edodes and A. bisporus. The length of IGS1 was varied: a 1. 1 kb product for P. djamor and P. salmo-neostramineus, a common product 1. 0 kb for the other Pleurotus isolates, 700 bp for H. serotina, 1. 3 kb for L. edodes and A. bisporus. Dendrogram based on PCR-RFLP of 28S rDNA 5'half, ITS and IGS1 suggested that H. serotina, L. edodes and A. bisporus could distinguish from Pleurotus, the isolates of Pleurotus were divided into five groups: P. tuber-re gium, P. djamor and P. salmoneostramineus, P. abalonus and P. cystiodisus, P. citri-nopileatus, the other isolates clustered together. P. abalonus and P. cystidiosus were the same species, as well as P. djamor and P. salmoneostramineus, P. pulmonarius and P. sajor-caju. rDNA PCR-RFLP was more suitable for taxonomy and phylogenetic relationships of Pleurotus.
【Fund】: Supported by National Natural Science Foundation of China
【CateGory Index】: Q949
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