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《Journal of Huazhong Agricultural》 2004-03
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Efficacy of Immersion with Vibrio harveyi Bacterin in Large Yellow Croaker,Pseudodciaena crocea

Mao Zhijuan 1) Lou Dan 1) Yang Bo 2)Wu Jianjun 2)Wu Xiongfei 1) Kenji Kawai 3)Chen Changfu 2,4) ( 1)Ningbo Fishery Research Institute, Zhejiang Province, Ningbo315012,China; 2)Fisheries College, Huazhong Agricultural University, Wuhan 430070, China; 3) Fish Disease Laboratory, Faculty of Agriculture, Kochi University, Nankoku, Kochi783-8502,Japan; 4)State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University,Wuhan 430070,China)  
Large yellow croaker(Pseudodciaena crocea)were immunized by immersion with formalin killed Vibrio harveyibacterin with the concentration of 1.0×10 8 cells/mL, 1.0×10 7 cells/mL and 1.0×10 6 cells/mL, and the treating time are 1 min, 10 min and 60 min respectively. After immersion, the fish was cultured in net cages. Relative survival percent (RPS)was monitored 8 to 18 weeks post immersion. It was showed that during 8~11 weeks post immersion, RPS was the highest,and it reached 95.0%~100.0%, and then slowly declined to 70.0%~80.0% during 16~18 weeks. Field statistics revealed a trend that the immunized groups infected with less diseases, diseases broke later, and the fish recovered sooner. The survival percent of the immunized groups reached 80.0%~95.0%, apparently higher than control groups. The fish at different life time, 40 days, 75 days and 100 days post hatch, respectively, were immunized and RPS was tested afterwards. The results indicated that 40 days old juvenile also acquired protective immunity by immersion. Booster immunization was carried out 56 days after the first immersion. High RPS level was detected during 8~16 weeks post boosting,which was much higher than the once treated group.
【Fund】: 浙江省宁波市重点科技计划项目 (0 0 2 5 0 2 );; 国家自然科学基金项目 (3 0 2 710 3 2 );; 华中农业大学农业微生物学国家重点实验室项目资助
【CateGory Index】: S942.5
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【References】
Chinese Journal Full-text Database 5 Hits
1 Gan Lingling;Wang Weifang;Gao Chunren;Lei Jilin;Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences;Fishery Administration Bureau of Fushun;;The preparation and application of inactivated vaccine and its antiserum from eight common pathogens of Flatfish[J];Engineering Sciences;2014-09
2 Mao Zhijuan 1) Lou Dan 1)Yan Maocang 2) Riichi Kusuda 3) Chen Changfu 2,4) ( 1)Ningbo FisheriesResearch Institute, Zhejiang Pro vince, Ningbo 315012, China; 2) Fishery College, Huazhong Agricultural Un iversity, Wuhan 430070, China, 3) Department of Marine Biotechnology,Fac ulty of Engineering, Fukuyama University,Fukuyama 729-02, Japan; 4)St ate Key Laboratory of Agricultural Microbiology, Huazhong Agricultural Univer si ty, Wuhan 430070, China);Primary Study on Comprehensive Prevention Strategy against Vibriosis of Large Yellow Croaker, Pseudosciaena crocea[J];Journal of Huazhong Agricultural;2004-05
3 PAN Xiao-yi1,SHEN Jin-yu1,YIN Wen-lin1,et al(1.Zhejiang Institute of Freshwater Fisheries, Huzhou 313001,China);Studies on the Optimal Culture Conditions of Vibrio harveyi BK-1 from Sparus macrocephalus[J];Journal of Zhejiang Ocean University;2005-03
4 Shen Jinyu Pan Xiaoyi Yin Wenlin Hao Guijie Cao Zheng (Zhejiang Institute of Freshwater Fisheries,Huzhou 313001);Studies on the Optimal Culture Conditions of Vibrio harveyi GYC1108-1 from Pseuclosciaena crocea[J];Biotechnology Bulletin;2009-S1
5 Xin Liu;Xueying Song;Xiaoping Zhang;Yinglun Han;Ting Zhu;Rong Xiao;Qingwei Li;School of Life Science, Liaoning Normal University;Lamprey Research Center, Liaoning Normal University;;Genetic basis of immune response of lymphocyte-like cells in the mucosal immune system of Lampetra japonica[J];Hereditas;2015-11
【Citations】
Chinese Journal Full-text Database 6 Hits
1 MAO Zhi juan 1,LIU Guo yong 2,CHEN Chang fu 2 (1 Ningbo Fishery Research Institute,Ningbo 315012; 2 Key Laboratory of Agricultural Microbiology, Ministry of Agriculture);Isolation and Identification of Pathogenic Bacteria Causing Ulcerosis in Large Yellow Croaker (Pseudosciaena crocea)[J];Journal of Anhui Agricultural University;2002-02
2 CHEN Yue-ying; QIAN Dong; SHEN Zhi-hua; SHEN Jin-yu;CAO Zheng; YIN Wen-lin; ZHANG Mian-ci(Zhejiang Institute of Freshwater Fisheries, Huzhou, 313001);STUDIES ON BATH IMMUNIZATION OF BACTERIN FOR FISH BACTERIAL SEPTICEMIA[J];OCEANOLOGIA ET LIMNOLOGIA SINICA;1998-06
3 LIN Ke-bing ; ZHOU Chen ; LIU Jia-fu; ZHOU Sheng-li; ZENG Zhi-nan; CHEN Mu (Fujian Fisheries Research Institute, Xiamen 361012 ) (Ningde Regain Frsheries Extension Station of Fujian Province, Ningde 352100);STUDIES ON THE PATHOGENIC BACTERIA OF Pseudosciaena crocea IN MARINE CAGE CULTURE[J];MARINE SCIENCES;1999-04
4 YANGXian Le (KeyLaboratoryofEcologyandPhysiologyinAquaculture ,MinistryofAgriculture ,ShanghaiFisheriesUniversity , 2 0 0 0 90 ) ZHOUJian Guang ,AIXiao Hui ,KEFu En (YangtzeRiverFisheriesInstitute ,Shashi 4 3 40 0 0 );INFLUENCEOFAGE ,SIZEANDNUTRITIONOF TRIONYXSINENSISONTHEIMMUNERESPONSE[J];JOURNAL OF FISHERIES OF CHINA;1999-04
5 Lin kebing; Zhou Chen; Liu Jiafu; Zhou Shengli; He Libin;Zeng Zhinan; Chen Mu (Fujian Fisheries Research Institute,Xiamen, 361012) (Ningde Region Fisheries Extension Station of Fujian Province,Ningde, 352100);Studies on pathogenic bacteria of Pseudoscieana crocea in marine cage culture[J];JOURNAL OF OCEANOGRAPHY IN TAIWAN STRAIT;1999-03
6 WANG Jun, SU Yong quan, ZHANG Zhao xia, LI Mo, ZHANG Jiao nan, YAN Qing pi (Dept. of Oceanography & Institute of Subtropical Oceanography, Xiamen Univ., Xiamen, 361005, China);Bacterial Pathogenetic biology of cultured Pseudosciaena crocea in southern Fujian[J];Journal of Xiamen University(Natural Science);2001-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Lei et al(College of Life Sciences,Hebei University,Baoding,Hebei 071002);Isolation,Identification and Drug Sensitivity Test of Suspect Pathogen of Diseased Cichlasoma spp.[J];Journal of Anhui Agricultural Sciences;2009-07
2 MAO Zhi juan 1,LIU Guo yong 2,CHEN Chang fu 2 (1 Ningbo Fishery Research Institute,Ningbo 315012; 2 Key Laboratory of Agricultural Microbiology, Ministry of Agriculture);Isolation and Identification of Pathogenic Bacteria Causing Ulcerosis in Large Yellow Croaker (Pseudosciaena crocea)[J];Journal of Anhui Agricultural University;2002-02
3 MAO Zhi-juan~1, LOU Dan~1,WU Jian-jun~2 (1.Ningbo Fisheries Research Institute, Ningbo 315012,China;2. Huazhong Agricultural University, Wuhang 430070,China);Studies on Culture Technological Conditions of Vibrio harveyi Y91301[J];Fisheries Science;2004-03
4 JIN Shan~1, WANG Guo-liang~1, ZHAO Qin-song~1, ZHEN Tian-lun~2, CHEN Yin-er~1 (1.Life Science and Technology Faculty, Ningbo University, Ningbo 315211, China; 2.Fisheries Technical Extension Station of Zhejiang Province, Hangzhou 310012, China);Epidemiology of Vibriosis in Large Yellow Croaker Pseudosciaena crocea (Richardson) in Marine Cage Culture[J];Fisheries Science;2005-01
5 ZHENG Tian-lun~1,WANG Guo-liang~2,JIN Shan~2(1.Zhejiang Fisheries Technical Extension Center, Hangzhou 310012,China; 2.Department of Oceanology and Fishery, Ningbo University, Ningbo, 315211,China);Administration of Chinese Herb Medicines in Control of Vibriosis in Cage-cultured Large Yellow Croaker[J];Fisheries Science;2005-02
6 TONG Guo-zhong 1,SHI Ya-su2,MA Jian-yin1,TAO li1(1.Medical College,Zhejiang Ocean University,Zhoushan 316000,China;2.Zhoushan Municipal Center for Disease Prevention and Control,Zhoushan 316000,China);Antibacterial Effect of Chinese Herbal Medicines on Pathogenic Bacteria in Large Yellow Croaker Pseudosciaena crocea in Marine Cage Culture[J];Fisheries Science;2007-08
7 LIU Lian-sheng1,2,YAN Mao-cang1,ZHAO Hai-quan2,CHEN Qian-ying2,MA Ai-min1,3,LIN Zhi-hua11.Zhejiang Mariculture Research Institute,Wenzhou 325005,China;2.College of Life Science,Anhui Agricultural University,Hefei 230036,China;3.College of Animal Sciences and Technology,Shandong Agricultural University,Tai'an 271018,China);The Optimal Culture Conditions of Bacterium Vibrio harveyi WG1702 from Clam Meretrix meretrix[J];Fisheries Science;2010-02
8 ZHANG Yu-gui(Sichuan Aquaculture Shcool,Hechuan,Chongqin 401520,China)HU Bo(Sichuan Aquaculture Shcool,Pixian,Sichuan 611730,China)HUANG Xiao-li,WANG Kai-yu College of Veterinary Medicine,Sichuan Agriculturel University,Ya'an,Sichuan 625014,Chin;Study on the Pathogen and Drug Susceptibility of Surface Ulcer of Hybrid Sturgeon[J];Journal of Yangtze University(Natural Science Edition);2011-06
9 PAN Xiao-yi,SHEN Jin-yu,YIN Wen-lin,CHAO Zheng (Key Laboratory of Fish Health and Immunology,Chinese Academy of Fishery Science,Zhejiang Institute of Freshwater Fisheries,Huzhou 313001,China);Vibriosis and their mechanisms in aquatic animals[J];Journal of Dalian Fisheries University;2006-03
10 SHEN Jin-yu1,XU Wen-jun2,YIN Wen-lin1,CAO Zheng1,PAN Xiao-yi1,SHI Hui2,HAO Gui-jie1(1.Zhejiang Institute of Freshwater Fisheries,Huzhou 313001,China;2.Zhejiang Institute of Marine Fisheries,Zhoushan 316100,China);The use of the vaccine derived from inactivated pathogenic Vibrio harvey strain GYC1108-1 in cage-cultivated large yellow croaker Pseudosciaena crocea[J];Journal of Dalian Ocean University;2010-03
China Proceedings of conference Full-text Database 1 Hits
1 HAO Guijie, SHEN Jinyu, PAN Xiaoyi, YIN Wenlin, CAO Zheng, (Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China);Preparation and Immunogenicity of Extracellular Proteinase from Vibrio Harveyi GYC1108-1[A];[C];2010
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 QIN Guo-min et al (Key Laboratory of Oceanic Biotechnology of Jiangsu Province,Huaihai College of Technology,Lianyungang,Jiangsu 222005);Infection of Aeromonas veronii from Diseased Cyprinus carpio L.and the Biological Characteristics of Its Pathogen[J];Journal of Anhui Agricultural Sciences;2008-19
2 MAO Zhi juan 1,LIU Guo yong 2,CHEN Chang fu 2 (1 Ningbo Fishery Research Institute,Ningbo 315012; 2 Key Laboratory of Agricultural Microbiology, Ministry of Agriculture);Isolation and Identification of Pathogenic Bacteria Causing Ulcerosis in Large Yellow Croaker (Pseudosciaena crocea)[J];Journal of Anhui Agricultural University;2002-02
3 LI Ya Nan (Department of Biotechnology, College of Life Sciences, Zhejiang University, Hangzhou?310012, China);ANALYSIS ON IMMUNOGLOBULIN INDUCED BY INOCULATION OF AEROMONAS HYDROPHILA IN GRASS CARP (CTENOPHARYNGODEN IDELLUS CUVIER ET VALENCINNES) [J];Acta Zoologica Sinica;2001-02
4 ZHU Zhuang-chun,SHI Xiang-guo,ZHANG Shu-jie,JIANG Guang-jiang,XING Zhao-bin,ZHAO Ya-long,LI Zhan-jun,WU Peng(Department of Life Science,North China Coal Medical College,Tangshan 063000,China);The Pathogenic Bacteria of the Ascites in Japanese Flounder(Paralichthys olivaceus)[J];Fisheries Science;2006-07
5 SUN Yi-min1,2,LI Nan1,2,SONG Jie2,LI Xiao-yue2,SHEN Hong-qi3,WANG Feng-min3,LI Quan-zhen3,CAO Jie-ying3,ZHAO Bao-hua2 1.Hebei Chemical Engineering and Pharmaceutical College,Shijiazhuang 050026,China;2.College of Life Science,Hebei Normal University,Shijiazhuang 050016,China;3.Department of Diseases Supervision,Hebei Fisheries Bureau,Shijiazhuang 050011,China;Isolation and Identification of Bacterial Pathogen Aeromonas hydrophila and Development of the Inactivated Vaccine in Japanese Flounder Paralichthys olivaceus Diseased with Ascites[J];Fisheries Science;2009-11
6 YU Xiao-li,CHEN Ming,LI Chao,LI Li-ping,LEI Ai-ying, ZHONG Mu-ni,LIANG Wan-wen(Guangxi Institute of Fisheries,Nanning 530021,China);Channel catfish Ictalurus punctatus outbreak infected by bacterium Streptococcus iniae[J];Journal of Dalian Fisheries University;2008-03
7 YU Lan-ping,WANG Bin, LI Yan,HU Liang,ZHAO Feng-mei(Key Laboratory of Mariculture,Agriculture Ministry,PRC,Dalian Fisheries Univ.,Dalian 116023,China);Isolation and identification of haemorrihagic septicaemia pathogen from cultivated turbot Scophthalmus maximus[J];Journal of Dalian Fisheries University;2008-05
8 PANG Xiao-Wen1,QIN Hong2,SHI Jian-Guo1 1Department of Pathology and Pathophysiology,2Department of Anatomy and Histology and Embryology,School of Basic Medicine,Fourth Military Medical University,Xi'an 710033,China;Preparation and characterization of monoclonal antibody against Vibrio vulnificus[J];Journal of the Fourth Military Medical University;2008-06
9 Chen Changfu;Mao Zhi Juan;and Wang Min(Fishery College,Huazhong Agricultural University,Wuhan,430070);CHARACTERISTICS OF EDWARDSIELLA FUJIANESIS TO THREE ANTIBOTIC[J];FRESHWATER FISHERIES;1996-03
10 HeLu ZengLingbing YangXianle (Yangtze River Institute of Fisheries,Chinese Academy of Fishery Sciences,Jingzhou 434000);Immune response of Silver prussian Carp to the Aeromonas hydrophila bacterin[J];FRESHWATER FISHERIES;1999-01
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 LUO Dian-hui,WANG Wen-yan,YUE Jun-yang,FANG Bai-shan (Department of Bioengineering and Biotechnology,Huaqiao University,Xiamen 361021,China);Culture Conditions of Vibrio fischer[J];Environmental Science & Technology;2010-08
2 HANG Wan-hong,CHEN Yue-zhong(Abalone institute of Zhangpu county,Zhangpu 363205,China;Fisheries research institute of Fujian,Xiamen 361012,China);A Primary Study of Prevention and Cure of Penaeid Shrimp Luminous Disease by Using Biological Control Methods[J];Journal of Fujian Fisheries;2007-02
3 SHU Bo2,HUANG Degui1,GUAN Wanchun1,PAN Changwang1,CHANG Yanxiang1(1.Wenzhou Medical College,Wenzhou 325035,China;2.Hunan Yongqing Envirojnmental Conservation Ltd-Co,Changsha 410005,China);Impact of PFOS on Growth and ECP Gene of Vibrio harveyi[J];Guangdong Trace Elements Science;2011-09
4 MAO Zhi-juan1 WANG Mei-zhen2 CHEN Ji-gang1 YANG Ji-fang1(1Biological and Environmental College,Zhejiang Wanli University,Ningbo 315100)(2Fishery College,Huazhong Agricultural University,Wuhan 430070);Isolation and characterization of Pseudomonas putida,a causative agent of gastroenteritis in black sea bream,Sparus macrocephalus[J];Progress in Fishery Sciences;2010-03
5 CHEN Yue-zhong1,HUANG Wan-hong2(1.Fisheries Research Institute of Fujian,Xiamen 361012,China;2.Zhangpu Institute of Abalone,Zhangpu 363205,China);Feasibility Study of Using Bacteriophage to Detect Penaeid Shrimp Luminous Disease Pathogen,Vibrio Harveyi[J];Journal of Jimei University(Natural Science);2007-04
6 MA Aimin1,2,YAN Maocang1,XIE Qilang1,CHEN Shaobo1,SHAN Lezhou1,CHANG Weishan2(1.Zhejiang Mariculture Research Institute,Wen zhou 325005,China;2.Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource,Wenzhou 325005,China;3.Department of Animal Sciences and Technology,Shandong Agricultural University,Tai’an,271018,China);Studies on the Optimum Culture Conditions of Vibrio splendidus M-1[J];Bulletin of Science and Technology;2009-06
7 ZHANG Xiao-Hua,ZHONG Ying-Bin,CHEN Ji-Xiang(College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China);The Biological Characteristics,Epidemiology and Detection Techniques of Vibrio Harveyi[J];Periodical of Ocean University of China;2007-05
8 JIANG Xiao1,2,REN Chun-hua1,HU Chao-qun1,LUO Peng1,CHEN Chang1,FENG Jing-bin1,2(1.South China Sea Institute of Oceanology,CAS,Guangzhou 510301,China;2.Graduate University of CAS,Beijing 100049,China);On dynamic change of Vibrio species in the Daya Bay using molecular identification method[J];Journal of Tropical Oceanography;2010-04
9 TAO Jia-fa,LAI Ying-tiao,PAN Hou-jun,SHI Cun-bin,WU Shu-qin(Pearl River Fisheries Research Institute,Chinese Academy of Fisheries Sciences,Guangdong Key Laboratory of Aquatic Animal Immune Techniques,Guangzhou 510380,China);Optimization Culture of Vibrio harveyi EcGY020401[J];Biotechnology;2010-05
10 Pan Xiaoyi Shen Jinyu Cao Zheng Yin Wenlin Hao Guijie Xu Yang Yao Jiayun(Key Laboratory of Fish Health and Immunology,Chinese Academy of Fishery Science, Zhejiang Institute of Freshwater Fisheries,Huzhou 313001);Phenotypic Cloning and Sequence Analysis of Extracellular Protease Gene of Vibrio harveyi[J];Biotechnology Bulletin;2010-10
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LIN Ke-bing ; ZHOU Chen ; LIU Jia-fu; ZHOU Sheng-li; ZENG Zhi-nan; CHEN Mu (Fujian Fisheries Research Institute, Xiamen 361012 ) (Ningde Regain Frsheries Extension Station of Fujian Province, Ningde 352100);STUDIES ON THE PATHOGENIC BACTERIA OF Pseudosciaena crocea IN MARINE CAGE CULTURE[J];MARINE SCIENCES;1999-04
2 Chen Yueying Ye Jinyun Shen Zhihua(Zhejiang Institute of Freshwater Fisheries, Huzhou 313001);EFFECT OF HENBANE AND TISSUE VACCINESUPPLEMENT ON THE GROWTH-PROMOTINGAND DISEASRESISTANCE OF GRASS CARP CTENOPHARYNGODON IDELLUS[J];;1994-02
3 Zheng Guoxing;Li He;Huang Ningyu;Yu Yeshao East China Sea Fisheries Research Institute, Shanghai Yang Jifang;Wu Youlu The second Institute of Oceanography, Hangzhou;CHARACTERISTICS OF VIBRIO ALGINOLYTICUS ISOLATED FROM DISEASED CLAM MERETRIX MERETRIX AND HISTOPATHOLOGICAL OBSERVATIONS ON DISEASED CLAM BY ELECTRON MICROSCOPE[J];Journal of Fisheries of China;1991-02
4 Chen Yueying, Qian Dong, Shen Zhihua, Shen Jinyu, Cao Zhen, Yin Wenlin and Zhang Nianci (Zhejiang Institute of Freshwater Fisherises, Huzhou 313001);PREPARATION OF OF BACTERIN FOR FISH BACTERIAL SEPTICEMIA[J];JOURNAL OF FISHERIES OF CHINA;1996-02
5 LI Jun, FENG Juan, LIU Xu,LI Qiu Feng ,XU Huai Shu (College of Marine Life Sciences, Ocean Univrsity of Qingdao, 266003) Norman Y S Woo (Department of Biology, The Chinese University of Hong Kong ,Shatin );VIBRIO ALGINOLYTICUS, A PATHOGEN OF SPARUS SARBA CULTURED IN HONG KONG[J];JOURNAL OF FISHERIES OF CHINA;1998-03
6 YANG XianLe (Key Lab of Ecology and Physiology in Aquaculture of the Ministry of Agriculture, Shanghai Fisheries University, 200090) HE Lu, AI XiaoHui,ZHOU JianGuang,KE FuEn (Yangtze River Fisheries Institute, Shashi 434000);A METHOD OF DETECTION OF ANTIBODY IN TRIONYX SINENSIS BY INDIRECT AGGLUTINATION OF RED CELL[J];JOURNAL OF FISHERIES OF CHINA;1999-01
7 Yin Zhan and Xu Bohai(Institute of Hydrobiology,the Cbinese Academy of Sciences,Wuhan 430072);STUDIES ON THE BACTERIOSIS OF FISHES[J];ACTA HYDROBIOLOGICA SINICA;1995-01
8 Lin kebing; Zhou Chen; Liu Jiafu; Zhou Shengli; He Libin;Zeng Zhinan; Chen Mu (Fujian Fisheries Research Institute,Xiamen, 361012) (Ningde Region Fisheries Extension Station of Fujian Province,Ningde, 352100);Studies on pathogenic bacteria of Pseudoscieana crocea in marine cage culture[J];JOURNAL OF OCEANOGRAPHY IN TAIWAN STRAIT;1999-03
9 Qian Dong Chen Yueying Shea Jiaym Shea Zhihu(Zhejiang Institute of Freshuttter Fisheries, Huxhou 313001);SEROGROUPS,VIRULENCE AND HEMOLYTIC ACTIVITY OF AEROMONAS HYDROPHILA WHICH CAUSED FISH BACTERIAL SEPTICAEMIA[J];Acta Microbiologica Sinica;1995-06
10 WANG Jun, SU Yong quan, ZHANG Zhao xia, LI Mo, ZHANG Jiao nan, YAN Qing pi (Dept. of Oceanography & Institute of Subtropical Oceanography, Xiamen Univ., Xiamen, 361005, China);Bacterial Pathogenetic biology of cultured Pseudosciaena crocea in southern Fujian[J];Journal of Xiamen University(Natural Science);2001-01
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