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《Journal of Huazhong Agricultural University》 2007-04
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Cytotoxicity Bioassay for Detection of Ciguatoxin

ZHENG Lu1,2) ZHU Hai1) GUO Ai-zhen2) FAN Fang1) LI Jin-feng1,2) ZHAO Fang1) YANG Xiao-xia1) HONG Xiao-liu1) MA Shu-mian1) SITU Cuihua1) (1) Food Inspection and Quarantine Center,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen 518067,China;2)State Key Laboratory of Agricultural Microbiology/College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China)  
The neuroblastoma cells were cultured in vitro.CTX at various doses,and ouabain and veratridine were added into the cultured cells.CTX can stimulate the sodium channel and induce cell death synergically with ouabain and veratridine.The culture was terminated at different time of coincubation and MTT,a viable cell dye,was used to measure quantitatively the viable cells in the form of optical density values at 570 nm.The linear relationship between P-CTX-1 dosage and optical density values at 570 nm was assayed.The results showed that the incubation time affected the linear relationship between P-CTX-1 dosage and cell viability.The regression equations at 10 h,15 h and 20 h were respectively y=-0.082 6x+0.923 0(R2=0.978 9),y=-0.044 3x+0.601 5(R2=0.962 1) and y=-0.020 4x+0.323 7(R2=0.928 1),where x means CTX dosage,while y means cell optical density values,and R means the regression coefficient.In conclusion,10 h is the best incubation time to detect ciguatoxin toxicity.The morphologic observation confirmed this result.
【Fund】: 国家质检总局科技项目(2006IK126)资助
【CateGory Index】: Q503
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