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《Journal of Huazhong Agricultural University》 2017-06
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Effects of a TetR family transcription factor Ms2583 on isoniazid sensitivity in Mycobacterium smegmatis

LIN Jian;HU Lihua;National Key Laboratory of Agricultural Microbiology,Center for Proteomics Research Huazhong Agricultural University;  
Bacteria use transcriptional regulation to respond to environmental stresses.However,only a few transcription factors regulating drug resistance have been reported.In this study,a transcriptional factor library of Mycobacterium smegmatis was screened on drug plates.A novel transcriptional factor,encoded by the MSMEG_2583(Ms2583)gene directly affecting M.smegmatis Isoniazid(INH)sensitivity was characterized.Over-expressing Ms2583 caused a significant increase in INH sensitivity of the transformed cells.Results of analyzing structural characteristics of Ms2583 showed that Ms2583 is a TetR family transcription factor.The bacterial one-hybrid assays and electrophoretic mobility shift assays demonstrated that Ms2583 could directly bind to its own promoter with specific interaction.These findings will provide a clue for studying the regulatory mechanism of TetR family transcription factor Ms2583.
【Fund】: 中国博士后科学基金项目(2016T90699)
【CateGory Index】: R378.91
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