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《JOURNAL HUAZHONG(CENTRAL CHINA) AGRICULTURAL UNIVERSITY》 1994-01
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OPTIMIZED CONDITIONS FOR PROTOPLAST FORMATION AND REGENERATION OF ASPERGILLUS NIGER AND TRICHODERMA RESSEI──Ⅱ.REGULARIZE SELECTION FOR CULTURE-AGE、ENZYMES MIXTURE RATIO、ENZYMIC TIME AND TEMPERATURE

Ai Yuncan;Zhao Xuehui;Wang Lushui(Huazhong Agricultural University,Wuhan 430070)  
The L9(34)experiment was done of culture-age,enzymes mixture(cellulase:snail exzyme:chitinase)ratio,enzymatic time and temperature with regard to effects on protoplasts formation and regeneration of A spergillus niger AMS11 and Trichoderma ressei QM9414.The optimized four parameters were 14 ̄16 hr./18 ̄20 Hr.,(8:4:2)/(5:2.5:2.5),32℃,2 ̄3hr.for AMS11 and QM9414 separately.
【Fund】: 国家自然科学基金
【CateGory Index】: Q938
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1 Ai Yunean Zhao Xuehui Wang Lushui (Huazhong Agricultural University, Wuhan 430070);OPTIMIZED CONDITIONS FOR PROTOPLAST FORMATION AND REGENERATION OF ASPERGILLUS NIGER AND TRICHODERMA REESEI——Ⅰ. SELECTION FOR STABLIZER BUFFER SYSTEM AND ENZYME MIXTURE[J];华中农业大学学报;1993-04
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1 LIU Ling-Ling;WANG Yong-Lin;XIONG Dian-Guang;XU Xin;TIAN Cheng-Ming;LIANG Ying-Mei;College of Forestry, Beijing Forestry University;Museum of Beijing Forestry University, Beijing Forestry University;;Genetic transformation system of Cytospora chrysosperma, the causal agent of poplar canker[J];微生物学通报;2017-10
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3 Chen Xiaoling;Chen Dong;Zhang Suisheng;Chen Ying;State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-Food Biorefinery,Guangxi Key Laboratory of Biorefinery, Guangxi Academy of Science;;Molecular Modification of Cre1 Gene Mediating Carbon Catabolite Repression in Trichoderma reesei[J];基因组学与应用生物学;2014-02
4 He Xiaohui;College of Life Science,Fujian Normal University;;Study on the Protoplast Preparation Conditions of Pleurotus eryngii[J];安徽农学通报;2014-06
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8 CHEN Xiao-ling,CHEN Dong,LU Zhi-long(State Key Laboratory of Non-food Biomass Enzyme Technology,National Engineering Research Center for Non-food Biorefinery,Guangxi Key Laboratory of Biorefinery,Guangxi Academy of Sciences,Nanning,Guangxi,530007,China);Molecular Modification of Endo-β-1,4-glucanase Gene Egl1 from Trichoderma reesei[J];广西科学;2011-03
9 LI Yao-zhong,WU Yong-jun,LI Da-na,ZHAN Shou-nian(Guizhou Key Laboratory of Agricultural Bioengineering,College of Life Science,Guizhou University,Guiyang,Guizhou 550025,China);Preliminary Study on Protoplast Formation and Regeneration of Bacillus Separated from Lobster Sauce[J];贵州农业科学;2010-05
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【Secondary References】
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6 TIAN Yuan1,YUAN Sheqiang2,BAI Xiaoqing1,YUAN Yaowu1(1.Department of Food Science and Technology,Agricultural University of Hebei,Baoding 071001,China;2.Science and Educate Development Agriculture Center,Agricultural University of Hebei,Baoding 071000,China);Production and regeneration conditions of Trichoderma koningii protoplast[J];中国酿造;2010-07
7 WANG Fei-fei,YUE Tian-li,YUAN Ya-hong,GAO Zhen-peng(College of Food Science and Engineering,Northwest A&F University,Yangling,Shaanxi 712100,China);Study on preparation and regeneration of protoplasts from CoQ_(10)-yield strains[J];西北农林科技大学学报(自然科学版);2009-03
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