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《JOURNAL HUAZHONG(CENTRAL CHINA) AGRICULTURAL UNIVERSITY》 1996-04
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INHIBITATION OF INTRODUCING ANTISENSE ACC OXIDASE GENE INTO TOMATO GENOME ON EXPRESSION OF ITS ENDOGENOUS GENE

Ye Zhibiao;Li Hanxia;Zheng Yonglian;Liu Houli(Huazhong Agricultural University, Wuhan 430070)  
The antisense cDNA clone of 1-aminocycloprapane-1-carboxylic acid (ACC)oxidase gene was ransformed into tomato genome mediated with Ti plasmid vector. The ACC oxidase activity and ethylene production rate were significantly inhibited in the leaves and fruits of transgenic plants. This shows that the expression of the endogenous ACC oxidase gene was down regulated.The inheritance of selective marker gene transferred is in the model of the simple Mendel's fashion in progenies of the self-crossed generation.
【Fund】: 国家自然科学基金 湖北省自然科学基金
【CateGory Index】: S641.203.2
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