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《JOURNAL HUAZHONG(CENTRAL CHINA) AGRICULTURAL UNIVERSITY》 1996-04
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SPECIFIC EXPRESSION OF EST CAT GOT ALLOZYME GENE IN MAIZE

Tie Shuanggui; Zheng Yonglian; Chen Xianchen; Liu Dingliag; Liu Xunjia (Huazhong Agricultural University,Wuhan 430070;Henan Anyang Agricultural Cnllege,Anyang 455000)  
The EST GOT and CAT allozyme of maize were analyzed by hrizontal starch gel eletrophorsis.The samples for seven treatments were gathered among five different genetic background materials of maize. The results indicated that the allpzyme pattern varied greatly between the different samples, and some tissues had a characteristic and distinguishing complement.Ontogenetic variations on the expression of allozyme gene were observed for coleoptil,sheath,green leaves, low temperature, mesocoty, roots, colluse. The EST pattern was changed greatly under different materials, but it was slightly altered under different tissues and treatments. The GOT and CAT allozyme profiles have more different expressions among the different tissues and treatments than among the different materials.No influence for above three kinds of allozyme gem's expression was found in cytoplasm of male sterile maize. The temporality and spatiality of allozyme gene's expression not only influenced by genetic factors,but also responsive to exogenously applied environmental signals (physical and chemical).The light induced gene of CAT2 to expression and the low temperature led gene of GOT1 to expression.
【Fund】: 国家自然科学基金 国际科学基金
【CateGory Index】: S513
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