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《JOURNAL HUAZHONG(CENTRAL CHINA) AGRICULTURAL UNIVERSITY》 1997-06
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LOCALIZATION AND IDENTIFICATION OF ENDOCRIN CELLS IN THE GASTROENTEROPANCREATIC SYSTEM OF TWO SPECIES OF STOMACH CONTAINING TELEOSTS

Fang Zhiping1)\ Pan Qiansheng1)\ Nie Xiuyun2)\ Bai Xuemei1)(1)Huazhong Agricultural University, Wuhan 430070;2)Fishery Institute of Hongsan, Wuhan 430070)  
By using 4 kinds of antisera raised against mammalian hormones: glucagon (Glu), gastrin (Gas), somatostatin (Som) and 5hydroxytryptamine (5HT), the gastroenteropancreatic (GEP) endocrine system of nile tilapia (Tilapia nilotica) and freshwater pomfret (Colossoma brachypomum) were studied with an immunocytochemical technique of streptavidin labelled peroxidase. The results showed that there were distribution of these amine precursor uptake and decarboxylaiton (APUD) cells in the digestive tract mucosa and pancreatic island in varying degress. Positive specific reaction of 3 kinds of antisera was found in the digestive tract of 2 fish species except glucagon antiserum. The 5HT positive reactive cells were the largest in the disgestive tract and the somatostatincells were the second. The gastrincells located only among the cardiac epithelial cells space. The glucagoncells were identified in the pancreatic island of the two fish species. The somatostatincells were only observed in the pancreatic island of nile tilapia. The morphologic characteristics of endocrine cells were described and their distribution feature were discussed.
【Fund】: 国家自然科学基金
【CateGory Index】: S91
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