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《JOURNAL OF HUAZHONG AGRICULTURAL》 1999-02
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STUDY ON ISOLATION,PURIFICATION AND CHARACTERISTICS OF POLYSACCHARIDE Le FROM THE FRUITBODY OF Lentinus Edodes

Yang Juan\ Wu Moucheng\ Zhang Shenghua\= (Huazhong Agricultural University , Wuhan 430070) \;  
After being extracted with hot water and precipitated with alcohol, proteins and colouring agent removed, then isolated by DEAE-cellulose and cellulose gel column chromatography, a pure polysaccharide Le was obtained from fruitbody of Lentinus edodes.Results of polyacrylamide gel electrophoresis and Sepharose CL6B column chromatography illustrate that Le is a homogeneous composition and polysaccharideprotein complex. Typical absorption peak of polysaccharide was shown in IR spectrum. Their compositions of monosaccharides, identified by gas chromatography, were arabinose, xylose, mannose, galactose and glucose in the molar ratio of 0.310.471.001.158.92.\;
【CateGory Index】: S646.12,Q539
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