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《Journal of Henan Institute of Science and Technology(Natural Sciences Edition)》 2006-02
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Cloning and Sequence of HB2 of Desulfurization Bacterium and Sequence Analysis of its 16S rDNA Fragrant

LIANG Yan-zhen,et al.(Luohe Academy of Agricultural Science,Luohe,Henan,462000,China)  
Microbial desulfurization technology shows great potential in energy industry and environmental protection.Total DNA was extracted by using NaAc method,and then 16S rDNA of HB2 was amplified from DNA extracted from meling water by eubacterium special primers.16S rDNA amplicons were purified with UNIQ-10 Column DNA purification system(Sangon,Shanghai) and was ligated into the vector pUCm-T(Sangon,Shanghai) following the manufacturer's instructions,added to competent cells of E.coli DH5α,and the cells were then plated on the selective medium containing IPTG,X-Gal and Ampicilin to screen for positive colonies.Positive colonies are detected by PCR and finally are sequenced.sequenced.According to DNA sequences data analysis,the sequence of the HB2 strain's 16S ribosomal DNA was found to be homogeneous at the rate of 98 % to the members of Pseudomonas in GenBank
【CateGory Index】: X701.3;Q78
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