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《Journal of Henan Institute of Science and Technology》 2009-01
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Establishment and application of colloidal gold immuno-chromatographicassay for detecting gE antibody of swine pseudo rabies virus

Bai Jing,Yu Xinhe,Bian Chuanzhou(Zhengzhou College of Animal Husbandry Engineering,Zhengzhou 450011,China)  
By PCR,a gE antigen epitope gene was obtained from the genome of swine pseudo rabies virus.We inserted the gene into an expression vector,,to yield the recombinant plasmid pET-22b(+)-gE.After induced by IPTG,a high expression of gE protein was obtained.The product of expression was specific to antisera against PRV by protein dot blot analysis.After purified,the gene-expressed gE acts as attenuated PRV antigen.Based-on sandwich ELISA technique,immunochromatographicassay test paper for detecting PRV is firstly developed in China.The method is more sensitive and specific than other methods and fit for clinical diagnosis of PRV.
【Fund】: 河南省重点科技攻关课题(072102130022)
【CateGory Index】: S858.28
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