The Anti-tumor Effect and Mechanism of Soluble Polysaccharides from Dendrobium Candidum
JIN Le-hong,LIU Chuan-fei,TANG Ting(College of Medicine and Healthcare Management,Hangzhou Normal University,Hangzhou,Zhejiang,310036,China)
Objective To study in vivo and in vitro tumor-inhibition of soluble polysaccharides from Dendrobium candidum(SPD) and to discuss the possible mechanism of its tumor-inhibition. Methods Tumor-inhibition of SPD was observed in mice inoculated with sarcoma S180 and in vitro human liver tumor cells SMMC27721.The superoxide dismutase(SOD) and malondialdehyde(MDA) in mice serum,and the change of index of thymus and spleen were detected and analyzed.The apoptosis of human neuroblastoma(SH2SY5Y) induced by SPD was observed and analyzed by Hoechst stain method. Results In vivo mice sarcoma S180 and in vitro human liver tumor cells SMMC27721 were inhibited significantly by SPD.The index of thymus and spleen and the activation of SOD was increased,but the level of MDA in mice serum was decreased by SPD.The apoptosis of SH-SY5Y was significantly promoted by SPD. Conclusion SPD has a remarked anti-tumor effect and its mechanism was probably related to promotion of immune organ function and oxidation-resistance.