The study of dose-response relationship of pulsed electromagnetic radiation on rat blood-brain-barrier
WANG Qi 1, REN Dong-qing 1, KUANG Fang 2, SUN Jing-min 2, WANG Jin 1, PANG Yi-bing 1, GUO Yao 1, GUO Guo-zheng 1. 1.Department of Radiation Medicine, Fourth Military Medical University, Xi'an 710032, China; 2.Institute of Neuroscience, Fourth Military Medical University, Xi'an 710032, China
Objective To investigate the dose-response relationship of electromagnetic radiation on rat blood-brain-barrier (BBB). Methods Evans blue (EB) was used on the rat by intravenous injection, then observed the effect of pulsed electromagnetic radiation (EMR) in different pulses through fluorescent microscope. Results EB could form fluorescent faculae in brain where the BBB permeability had changed; the number and area of fluorescent faculae increased along with pulse numbers (0-200 pulses). Fluorescent faculae in EMR group were distributed over the rat brain, especially in cerebral cortex, thalamus, hypothalamus, cerebellum, caudate putamen and medulla oblongata. Conclusions Pulsed EMR in different frequence could induce different quantitative change of BBB permeability in rat brain, at 200 pulses the change was the most obvious, cerebral BBB permeability changed obvious more than others.